pUC18T-mini-Tn7T-Apr-sfGFP vector (V016355)

LOCUS       62056_21955        5670 bp DNA     circular SYN 02-APR-2019
DEFINITION  Cloning vector pUC18T-mini-Tn7T-Apr-sfGFP, complete sequence.
ACCESSION   MH976505
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Ducas-Mowchun K, De Silva PM, Crisostomo L, Fernando DM, Chao TC, 
            Pelka P, Schweizer HP, Kumar A.
  TITLE     Next Generation of Tn7-based single copy insertion elements for use 
            in multi- and pandrug resistant strains of Acinetobacter baumannii
  JOURNAL   Appl. Environ. Microbiol. (2019) In press
  PUBMED    30902859
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Ducas-Mowchun K, De Silva PM, Kumar A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2018) Department of Microbiology, University of 
            Manitoba, 45, Chancellor Circle, Winnipeg, Manitoba R3T2N2, Canada
REFERENCE   3  (bases 1 to 5670)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol. (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-SEP-2018) Department of Microbiology, University of Manitoba, 
            45, Chancellor Circle, Winnipeg, Manitoba R3T2N2, Canada"
FEATURES             Location/Qualifiers
     source          1..5670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  382..580
                     /mobile_element_type="transposon:Tn7R"
                     /label=n7R
                     /note="right end of Tn7"
     primer_bind     585..601
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      629..723
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      826..912
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    958..1005
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1123..1923)
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     promoter        complement(2164..2192)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_feature    2233..2280
                     /note="FRT; Flp recombinase target site"
     misc_feature    2307..2355
                     /note="MCS; multiple cloning site"
     CDS             complement(2364..3077)
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
     protein_bind    complement(3108..3124)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3132..3160)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     misc_feature    3166..3178
                     /note="MCS; multiple cloning site"
     mobile_element  complement(3187..3352)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            complement(3511..3619)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(3851..4439)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4613..5470)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5471..5575)
                     /label=AmpR promoter