pCDNA3.1.2S vector (V016336)

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LOCUS       V016336                 9209 bp    DNA     circular SYN 12-OCT-2020
DEFINITION  Exported.
ACCESSION   V016336
VERSION     V016336
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9209)
  AUTHORS   Nie J, Li Q, Wu J, Zhao C, Hao H, Liu H, Zhang L, Nie L, Qin H, Wang
            M, Lu Q, Li X, Sun Q, Liu J, Fan C, Huang W, Xu M, Wang Y.
  TITLE     Establishment and validation of a pseudovirus neutralization assay
            for SARS-CoV-2
  JOURNAL   Emerg Microbes Infect 9 (1), 680-686 (2020)
   PUBMED   32207377
REFERENCE   2  (bases 1 to 9209)
  AUTHORS   Li Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2020) Division of HIV/AIDS and Sex-transmitted
            Virus Vaccines, National Institutes for Food and Drug Control, No.
            31 Huatuo Street, Daxing District, Beijing, Beijing 102629, China
REFERENCE   3  (bases 1 to 9209)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Emerg
            Microbes Infect"; date: "2020"; volume: "9"; issue: "1"; pages:
            "680-686"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (14-JUN-2020) Division of HIV/AIDS and Sex-transmitted Virus
            Vaccines, National Institutes for Food and Drug Control, No. 31
            Huatuo Street, Daxing District, Beijing, Beijing 102629, China"
FEATURES             Location/Qualifiers
     source          1..9209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             941..4759
                     /gene="S"
                     /label="Spike glycoprotein"
                     /note="Spike glycoprotein from Severe acute respiratory
                     syndrome coronavirus 2. Accession#: P0DTC2"
     polyA_signal    4806..5030
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5076..5504
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5518..5847
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5914..6705
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    6882..7015
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7052..7068)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7076..7092)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7100..7130)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7145..7166)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7454..8042)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8216..9073)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9074..9178)
                     /label="AmpR promoter"