pR6K-AKC vector (V016334)

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LOCUS       V016334                 2999 bp    DNA     circular SYN 06-JUN-2019
DEFINITION  Exported.
ACCESSION   V016334
VERSION     V016334
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Moore CL, Papa LJ III, Shoulders MD.
  TITLE     A Processive Protein Chimera Introduces Mutations across Defined DNA
            Regions In Vivo
  JOURNAL   J. Am. Chem. Soc. 140 (37), 11560-11564 (2018)
   PUBMED   29991261
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Moore CL, Papa LJ III, Shoulders MD.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2019) Chemistry, Massachusetts Institute of
            Technology, 77 Massachusetts Avenue, 16-573A, Cambridge, MA 02139,
            USA
REFERENCE   3  (bases 1 to 2999)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Am.
            Chem. Soc."; date: "2018"; volume: "140"; issue: "37"; pages:
            "11560-11564"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-APR-2019) Chemistry, Massachusetts Institute of Technology, 77
            Massachusetts Avenue, 16-573A, Cambridge, MA 02139, USA"
FEATURES             Location/Qualifiers
     source          1..2999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             23..325
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             368..739
                     /gene="rpsL"
                     /label="Small ribosomal subunit protein uS12"
                     /note="Small ribosomal subunit protein uS12 from Salmonella
                     paratyphi A (strain ATCC 9150 / SARB42). Accession#:
                     Q5PIW1"
     rep_origin      793..1181
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(1249..1911)
                     /label="amilCP"
                     /note="amilCP is a fluorescent protein published in 2008,
                     derived from Acropora millepora. It is reported to be a
                     somewhat slowly-maturing tetramer."
     regulatory      1918..1929
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
                     /regulatory_class="ribosome_binding_site"
     promoter        complement(1965..1995)
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     promoter        2073..2120
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             2139..2939
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"