pTrcHisA-GFP(tvLAA)-(teF)tvmv vector (V016330)

LOCUS       62056_21390        5826 bp DNA     circular SYN 25-MAR-2019
DEFINITION  Cloning vector pTrcHisA-GFP(tvLAA)-(teF)tvmv, complete sequence.
ACCESSION   MK258729
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Gao C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-DEC-2018) State Key Laboratory of Food Science and 
            Technology, Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su 
            214122, China
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (05-DEC-2018) State Key Laboratory of Food Science and Technology, 
            Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su 214122, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        193..222
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    230..246
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    263..332
                     /label=rrnG antiterminator
                     /note="antiterminator from the E. coli rrnG leader region
                     (Berg et al., 1989)"
     regulatory      368..372
                     /label=RBS
                     /note="RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             383..406
                     /label=minicistron
                     /note="synthetic cistron containing a ribosome binding site
                     (Shine-Dalgarno sequence), for enhancing the bacterial 
                     expression of a downstream cistron (Schoner, 1997)"
     CDS             425..442
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             455..1171
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    1178..1195
                     /label=TVMVp cleave site
                     /note="TVMVp cleave site"
     misc_feature    1202..1234
                     /label=ssrA tag
                     /note="ssrA tag"
     regulatory      1257..1268
                     /label=b0034 RBS
                     /note="b0034 RBS"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    1278..1295
                     /label=TEVp cleave site
                     /note="TEVp cleave site"
     misc_feature    1296..1310
                     /label=F degron
                     /note="F degron"
     terminator      2179..2265
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2357..2384
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2404..2495
                     /label=AmpR promoter
     CDS             2496..3353
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3527..4115
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4301..4441)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        4627..4704
                     /label=lacI promoter
     CDS             4705..5784
                     /label=lacI
                     /note="lac repressor"