pSC101-CsrA-Bio-phaC-pct-GP44-AAV vector (V016318)

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LOCUS       V016318                 7392 bp    DNA     circular SYN 16-MAY-2020
DEFINITION  Exported.
ACCESSION   V016318
VERSION     V016318
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7392)
  AUTHORS   Guo L.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2019) Jiangnan University, State Key Laboratory of
            Food Science and Technology, 1800 Lihu Avenue, Wuxi, Jiangsu 214122,
            China
REFERENCE   2  (bases 1 to 7392)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (28-OCT-2019) Jiangnan University, State Key Laboratory of Food
            Science and Technology, 1800 Lihu Avenue, Wuxi, Jiangsu 214122,
            China"
FEATURES             Location/Qualifiers
     source          1..7392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(100..129)
                     /label="T3Te terminator"
                     /note="phage T3 early transcription terminator"
     CDS             complement(185..367)
                     /gene="csrA"
                     /label="Carbon storage regulator"
                     /note="Carbon storage regulator from Escherichia coli
                     (strain K12). Accession#: P69913"
     promoter        complement(450..484)
                     /label="J23119 promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119)"
     terminator      501..572
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     gene            667..2346
                     /gene="phaC"
                     /label="phaC"
     CDS             667..2346
                     /codon_start=1
                     /transl_table=11
                     /gene="phaC"
                     /product="polyhydroxyalkanoate synthase"
                     /label="phaC"
                     /protein_id="QJR97798.1"
                     /translation="MSNKNSDDLNRQASENTLGLNPVIGLRGKDLLTSARMVLTQAIKQ
                     PIHSVKHVAHFGIELKNVMFGKSKLQPESDDRRFNDPAWSQNPLYKRYLQTYLAWRKEL
                     HDWIGNSKLSEQDINRAHFVITLMTEAMAPTNSAANPAAVKRFFETGGKSLLDGLTHLA
                     KDLVNNGGMPSQVDMGAFEVGKSLGTTEGAVVFRNDVLELIQYRPTTEQVHERPLLVVP
                     PQINKFYVFDLSPDKSLARFCLSNNQQTFIVSWRNPTKAQREWGLSTYIDALKEAVDVV
                     SAITGSKDINMLGACSGGITCTALLGHYAALGEKKVNALTLLVTVLDTTLDSQVALFVD
                     EKTLEAAKRHSYQAGVLEGRDMAKVFAWMRPNDLIWNYWVNNYLLGNEPPVFDILFWNN
                     DTTRLPAAFHGDLIEMFKNNPLVRANALEVSGTPIDLKQVTADIYSLAGTNDHITPWKS
                     CYKSAQLFGGKVEFVLSSSGHIKSILNPPGNPKSRYMTSTDMPATANEWQENSTKHTDS
                     WWLHWQAWQAERSGKLKKSPTSLGNKAYPSGEAAPGTYVHER"
     gene            2374..3927
                     /gene="pct"
                     /label="pct"
     CDS             2374..3927
                     /codon_start=1
                     /transl_table=11
                     /gene="pct"
                     /product="propionyl-CoA transferase"
                     /label="pct"
                     /note="PCT"
                     /protein_id="QJR97799.1"
                     /translation="MRKVEIITAEQAAQLVKDNDTITSIGFVSSAHPEALTKALEKRFL
                     DTNTPQNLTYIYAGSQDKRDGRAAEHLAHTGLLKRAIIGHWQTVPAIGKLAVENKIEAY
                     NFSQGTLVHWFRALAGHKLGVFTDIGLETFLDPRQLGGKLNDVTKEDLVKLIEVDGHEQ
                     LFYPTFPVNVAFLRGTYADESGNITMDEEIGPFESTSVAQAVHNCGGKVVVQVKDVVAH
                     GSLDPRMVKIPGIYVDYVVVAAPEDHQQTYDCEYDPSLSGEHRAPEGATDAALPMSAKK
                     IIGRRGALELTENAVVNLGVGAPEYVASVAGEEGIADTITLTVEGGAIGGVPQGGARFG
                     SSRNADAIIDHTYQFDFYDGGGLDIAYLGLAQCDGSGNINVSKFGTNVAGCGGFPNISQ
                     QTPNVYFCGTFTAGGLKIAVEDGKVKILQEGKAKKFIKAVDQITFNGSYAARNGKHVLY
                     ITERCVFELTKEGLKLIEVAPGIDIEKDILAHMDFKPIIDNPKLMDARLFQDGPMGLKK
                     "
     gene            3952..4167
                     /gene="gp44"
                     /label="gp44"
     CDS             3952..4167
                     /codon_start=1
                     /transl_table=11
                     /gene="gp44"
                     /product="recombination directionality factor"
                     /label="gp44"
                     /note="Gp44"
                     /protein_id="QJR97800.1"
                     /translation="MSEVLVSASYEGYESNSINFTEINNIVKERFKKIDEVERKKRAET
                     FNKKYEVTKELVNGHLREIIIPRRLIK"
     terminator      4251..4282
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     terminator      4407..4501
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     rep_origin      4993..5215
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             5263..6210
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     CDS             6502..7359
                     /label="AmpR"
                     /note="beta-lactamase"
     terminator      7373..7392
                     /label="his operon terminator"
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during
                     synthesis. Its efficiency has not been determined."