pXJ070 vector (V016284)

LOCUS       62056_22810        7170 bp DNA     circular SYN 16-MAY-2020
DEFINITION  Cloning vector pXJ070, complete sequence.
ACCESSION   MT084772
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Wang Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-FEB-2020) Single-Cell Center, Qingdao Institute of 
            Bioenergy and Bioprocess Technology, No. 189 Songling Road, Laoshan 
            District, Qingdao, Shandong Province, Qindao, Shandong 266000, China
REFERENCE   2  (bases 1 to 7170)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (20-FEB-2020) Single-Cell Center, Qingdao Institute of Bioenergy and
            Bioprocess Technology, No. 189 Songling Road, Laoshan District, 
            Qingdao, Shandong Province, Qindao, Shandong 266000, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     regulatory      159..558
                     /regulatory_class="terminator"
     CDS             559..930
                     /label=BleoR
                     /note="antibiotic-binding protein"
     CDS             931..948
                     /label=6xHis
                     /note="6xHis affinity tag"
     regulatory      952..1459
                     /regulatory_class="terminator"
     CDS             complement(2008..2040)
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             complement(2044..2061)
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             complement(2071..2088)
                     /label=6xHis
                     /note="6xHis affinity tag"
     RBS             complement(2107..2129)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(2144..2168)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2169..2187)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2496..2573
                     /label=lacI promoter
     CDS             2574..3653
                     /label=lacI
                     /note="lac repressor"
     protein_bind    3669..3690
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             4465..4653
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    4758..4900
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5086..5674)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             5796..6608
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(6704..7159)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"