pBS-PtPDS1-M1 vector (V016283)

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LOCUS       62056_4185        5330 bp DNA     circular SYN 12-JUN-2019
DEFINITION  Transformation vector pBS-PtPDS1-M1, complete sequence.
ACCESSION   MK645853
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Taparia Y, Zarka A, Leu S, Zarivach R, Boussiba S, Khozin-Goldberg 
            I.
  TITLE     A novel endogenous selection marker for the diatom Phaeodactylum 
            tricornutum based on a unique mutation in phytoene desaturase 1
  JOURNAL   Sci Rep 9 (1), 8217 (2019)
  PUBMED    31160749
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Taparia Y, Leu S, Zarka A, Zarivach R, Khozin-Goldberg I, Boussiba 
            S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2019) Albert Katz International School for Desert 
            Research, Ben-Gurion University of The Negev, French Associates 
            Institute for Agriculture and Biotechnology, Sede Boqer, Israel 
            8499000, Israel
REFERENCE   3  (bases 1 to 5330)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep"; 
            date: "2019"; volume: "9"; issue: "1"; pages: "8217"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAR-2019) Albert Katz International School for Desert Research, 
            Ben-Gurion University of The Negev, French Associates Institute for 
            Agriculture and Biotechnology, Sede Boqer, Israel 8499000, Israel"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      731..976
                     /regulatory_class="promoter"
     CDS             1243..1257
                     /label=SNAC tag
                     /note="tag for protein cleavage using Ni2+ ion (Dang et
                     al., 2019)"
     regulatory      2852..3093
                     /regulatory_class="terminator"
     promoter        complement(3142..3160)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3181..3197)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3205..3221)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3229..3259)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3274..3295)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3583..4171)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4345..5202)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5203..5307)
                     /label=AmpR promoter