PB-Gal4-Teton-cis vector (V016282)

LOCUS       62056_3360        8224 bp DNA     circular SYN 01-SEP-2019
DEFINITION  Cloning vector PB-Gal4-Teton-cis, complete sequence.
ACCESSION   MK422200
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8224)
  AUTHORS   Chen B.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2019) Stem Cell Center, Institute of Blood 
REFERENCE   2  (bases 1 to 8224)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (17-JAN-2019) Stem Cell Center, Institute of Blood Transfusion, 
            Chinese Academy of Medical Sciences "
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1373..1391
                     /label=tet operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        1581..1619
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             1684..2349
                     /label=CopGFP
                     /note="green fluorescent protein 2 from Pontellina plumata,
                     also known as ppluGFP2 (Shagin et al., 2004)"
     CDS             2419..2472
                     /label=T2A
                     /note="2A peptide from Thosea asigna virus capsid protein"
     regulatory      2529..2653
                     /note="UAS; upstream activating sequences"
                     /regulatory_class="transcriptional_cis_regulatory_region"
     CDS             2970..3677
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    3678..3743
                     /note="P2A; 2A peptide"
     polyA_signal    3792..4016
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        4068..4397
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        4445..4492
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             4511..4882
                     /label=BleoR
                     /note="antibiotic-binding protein"
     polyA_signal    5015..5136
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6203..6219)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6227..6243)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6251..6281)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6296..6317)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6605..7193)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7367..8224)
                     /label=AmpR
                     /note="beta-lactamase"