pUASGal80-PUbEGFP-attB vector (V016278)

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LOCUS       62056_21795        9289 bp DNA     circular SYN 26-FEB-2020
DEFINITION  Cloning vector pUASGal80-PUbEGFP-attB, complete sequence.
ACCESSION   MN830806
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9289)
  AUTHORS   Webster SH, Vella MR, Scott MJ.
  TITLE     Development and testing of a novel Killer-Rescue self-limiting gene 
            drive system in Drosophila melanogaster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9289)
  AUTHORS   Webster SH, Vella MR, Scott MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2019) Entomology and Plant Pathology, North 
            Carolina State University, 112 Derieux Place, 1546 Thomas Hall, 
            Raleigh, NC 27607, USA
REFERENCE   3  (bases 1 to 9289)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-DEC-2019) Entomology and Plant Pathology, North Carolina State 
            University, 112 Derieux Place, 1546 Thomas Hall, Raleigh, NC 27607, 
            USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        243..2051
                     /label=Polyubiquitin
                     /note="Promoter of polyubiqutin gene of Drosophila
                     melanogaster"
     CDS             2076..2096
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             2280..2996
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    3122..3243
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(3309..3327)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3358..3376
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3415..3433)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3440..3456)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(3597..4052)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4079..4183
                     /label=AmpR promoter
     CDS             4184..5041
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5215..5803
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6091..6112
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6127..6157
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6165..6181
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6189..6205
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6226..6244
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(6463..6532)
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     protein_bind    6669..6763
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        6922..7160
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     intron          7175..7241
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     regulatory      7264..7284
                     /label=syn21 translation enhancer
                     /note="syn21 translation enhancer"
                     /regulatory_class="other"
     CDS             7285..8589
                     /label=GAL80
                     /note="transcriptional repressor that inhibits GAL4"
     regulatory      8598..9264
                     /label=p10 pA
                     /note="p10 pA"
                     /regulatory_class="polyA_signal_sequence"