pK2GW7_HSP_FT vector (V016272)

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LOCUS       V016272                 9470 bp    DNA     circular SYN 15-MAR-2020
DEFINITION  Exported.
ACCESSION   V016272
VERSION     V016272
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9470)
  AUTHORS   Kersten B, Leite Montalvao AP, Hoenicka H, Mueller NA, Vettori C,
            Paffetti D, Fladung M.
  TITLE     Resequencing of two transgenic early-flowering poplar lines
            confirmed integration of a single T-DNA copy in both lines without
            any unexpected additional integration of T-DNA vector fragments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9470)
  AUTHORS   Kersten B, Hoenicka H, Fladung M, Nilsson O.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2019) Genome Research, Thuenen Institute of Forest
            Genetics, Sieker Landstrasse 2, Grosshansdorf 22927, Germany
REFERENCE   3  (bases 1 to 9470)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: SeqMan Pro v. v15.1
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-AUG-2019) Genome Research, Thuenen Institute of Forest Genetics,
            Sieker Landstrasse 2, Grosshansdorf 22927, Germany"
            The construction of the vector was described by Huang T. et al.
            (Science, 2005). The nucleotide sequence of the vector backbone is
            according to the binary GATEWAY destination vector pK2GW7 (Genbank
            accession JC487359). The vector  pK2GW7 was derived from plasmid
            pPZP200 (Genbank accession U10460; Hajdukiewicz P. et al., Plant
            Mol. Biol. 1994).
FEATURES             Location/Qualifiers
     source          1..9470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    239..263
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(356..608)
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(651..1442)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(1476..1655)
                     /label="NOS promoter"
                     /note="nopaline synthase promoter"
     regulatory      1773..2238
                     /note="HSP promoter from the soybean Gmhsp 17.6L heat-shock
                     protein gene"
                     /regulatory_class="promoter"
     protein_bind    2251..2275
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2276..2800
                     /gene="FT"
                     /label="Protein FLOWERING LOCUS T"
                     /note="Protein FLOWERING LOCUS T from Arabidopsis thaliana.
                     Accession#: Q9SXZ2"
     protein_bind    complement(2804..2828)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2838..3063
                     /label="CAMV 35S terminator (terminal 35S)"
                     /note="CAMV 35S terminator (terminal 35S)"
                     /regulatory_class="terminator"
     misc_feature    3143..3167
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4498..5124
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             5561..6625
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      6694..6888
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7232..7372
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7558..8146)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8393..9181)
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"