pNRVL-LEU2-6kbp vector (V016270)

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LOCUS       V016270                 8333 bp    DNA     circular SYN 20-MAR-2021
DEFINITION  Exported.
ACCESSION   V016270
VERSION     V016270
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Nemeth TM.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2020) Department of Microbiology, University of
            Szeged, Kozep fasor 52, Szeged, Csongrad 6726, Hungary
REFERENCE   2  (bases 1 to 8333)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. v11
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (27-AUG-2020) Department of Microbiology, University of Szeged,
            Kozep fasor 52, Szeged, Csongrad 6726, Hungary"
FEATURES             Location/Qualifiers
     source          1..8333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    570..575
                     /label="StuI site for cassette release"
                     /note="StuI site for cassette release"
     misc_feature    576..1039
                     /note="Site of recombination; CpNEUT5LUpup from C.
                     parapsilosis"
     misc_feature    1040..1047
                     /label="NotI site for selection marker ligation"
                     /note="NotI site for selection marker ligation"
     regulatory      1096..1943
                     /label="Candida maltosa LEU2 promoter"
                     /note="Candida maltosa LEU2 promoter"
                     /regulatory_class="promoter"
     CDS             1944..3062
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     regulatory      3066..3280
                     /label="Candida maltosa LEU2 terminator"
                     /note="Candida maltosa LEU2 terminator"
                     /regulatory_class="terminator"
     misc_feature    3314..3319
                     /note="BssHII site for selection marker and insert
                     ligation"
     misc_feature    3320..6091
                     /note="Candida albicans intergenic region;
                     Ca22chrRB_C_albicans_SC5314:2080135-2082906"
     misc_feature    6092..6097
                     /note="BssHII site for selection marker and insert
                     ligation"
     misc_feature    6105..6110
                     /label="XhoI site for insert ligation"
                     /note="XhoI site for insert ligation"
     misc_feature    6111..6561
                     /note="Site of recombination; CpNEUT5LDowndown from C.
                     parapsilosis"
     misc_feature    6562..6567
                     /label="StuI site for cassette release"
                     /note="StuI site for cassette release"
     promoter        complement(6575..6593)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6598..6614)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             6727..7533
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      7683..8271
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"