pSN.5_mTFP1 vector (V016257)

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LOCUS       62056_20040        9326 bp DNA     circular SYN 18-NOV-2021
DEFINITION  Cloning vector pSN.5_mTFP1, complete sequence.
ACCESSION   MT891156
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9326)
  AUTHORS   Pasin F, Kulasekaran S, Natale P, Simon-Mateo C, Garcia JA.
  TITLE     Rapid fluorescent reporter quantification by leaf disc analysis and 
            its application in plant-virus studies
  JOURNAL   Plant Methods 10, 22 (2014)
  PUBMED    25053970
REFERENCE   2  (bases 1 to 9326)
  AUTHORS   Pasin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2020) Department of Plant Molecular Genetics, 
            Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 9326)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods 10, 22 (2014)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-AUG-2020) Department of Plant Molecular Genetics, Centro 
            Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid 
            28049, Spain"
FEATURES             Location/Qualifiers
     source          1..9326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        407..1076
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     5'UTR           1076..1221
                     /label=Plum pox virus 5'UTR
                     /note="Plum pox virus 5'UTR"
     CDS             1222..1929
                     /label=mTFP1
                     /note="monomeric teal (cyan) fluorescent protein (Ai et
                     al., 2006)"
     CDS             1930..1944
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     CDS             1954..1977
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     3'UTR           2151..2367
                     /label=Plum pox virus 3'UTR
                     /note="Plum pox virus 3'UTR"
     terminator      2449..2701
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2792..2816
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4116..4742
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             5179..6243
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      6312..6506
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6850..6990
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7176..7764)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8011..8799)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"