pET28A-blaSME-1 vector (V016250)

LOCUS       V016250                 6574 bp    DNA     circular SYN 06-MAY-2019
DEFINITION  Exported.
ACCESSION   V016250
VERSION     V016250
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6574)
  AUTHORS   Hu XM, Yan XH, Yang B, Wang Q, Zheng L.
  TITLE     Full-length Real-time PCR for Rapid Detection of Seventeen
            Carbapenemase Genes in Gram-negative bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6574)
  AUTHORS   Hu XM, Yan XH, Yang B, Wang Q, Zheng L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2019) Department of Laboratory Medicine, Nanfang
            Hospital, Southern Medical University, No. 1383, Guangzhou Dadao
            Bei, Guangzhou, Guangdong 510515, China
REFERENCE   3  (bases 1 to 6574)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-APR-2019) Department of Laboratory Medicine, Nanfang Hospital,
            Southern Medical University, No. 1383, Guangzhou Dadao Bei,
            Guangzhou, Guangdong 510515, China"
FEATURES             Location/Qualifiers
     source          1..6574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(563..1375)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1497..2085
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2271..2413)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2518..2706)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(3481..3502)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3518..4597)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(4598..4675)
                     /label="lacI promoter"
     promoter        4984..5002
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5003..5027
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5042..5064
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5083..5100
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             5110..5127
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     CDS             5131..5163
                     /label="T7 tag (gene 10 leader)"
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             5300..6181
                     /gene="smeA"
                     /label="Carbapenem-hydrolyzing beta-lactamase Sme-1"
                     /note="Carbapenem-hydrolyzing beta-lactamase Sme-1 from
                     Serratia marcescens. Accession#: P52682"
     CDS             6418..6435
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      6502..6549
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"