pVG304_e1-y1 vector (V016248)

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LOCUS       62056_22555        8976 bp DNA     circular SYN 02-NOV-2019
DEFINITION  Expression vector pVG304_e1-y1, complete sequence.
ACCESSION   MN551094
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8976)
  AUTHORS   Lopez del Amo V Sr., Bishop AL Sr., Sanchez C HM, Bennett JB, Feng 
            X, Marshall JM, Bier E, Gantz VM.
  TITLE     Trans-complementing split-gene drive system provides flexible 
            application for safer laboratory investigation and potential field 
            deployment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8976)
  AUTHORS   Lopez del Amo V Sr., Bishop AL Sr., Sanchez C HM, Bennett JB, Feng 
            X, Marshall JM, Bier E, Gantz VM.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2019) Biological Sciences, University of 
            California San Diego, 9500 Gilman Dr, Bonner Hall 4305, La Jolla, CA
            92093, USA
REFERENCE   3  (bases 1 to 8976)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-OCT-2019) Biological Sciences, University of California San 
            Diego, 9500 Gilman Dr, Bonner Hall 4305, La Jolla, CA 92093, USA"
FEATURES             Location/Qualifiers
     source          1..8976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    877..898
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        913..943
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1009..1027
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    1107..2116
                     /label=ebony homology arm
                     /note="ebony homology arm"
     promoter        2123..2919
                     /label=dU6-1 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-1
                     snRNA (Port et al., 2014)"
     misc_feature    2919..2938
                     /label=e1 gRNA
                     /note="e1 gRNA"
     misc_RNA        2939..3014
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        3015..3435
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3
                     snRNA (Port et al., 2014)"
     misc_feature    3435..3454
                     /label=y1 gRNA
                     /note="y1 gRNA"
     misc_feature    3455..3530
                     /label=gRNA scaffold
                     /note="gRNA scaffold"
     misc_feature    3537..3838
                     /label=U6-3_3'UTR
                     /note="U6-3_3'UTR"
     polyA_signal    complement(3865..3986)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4112..4828)
                     /label=EGFP
                     /note="enhanced GFP"
     regulatory      4883..5007
                     /label=Hsp70 promoter
                     /note="Hsp70 promoter"
                     /regulatory_class="promoter"
     misc_feature    5039..5082
                     /label=3xP3 eye promoter
                     /note="3xP3 eye promoter"
     misc_feature    5106..6109
                     /label=ebony homology arm
                     /note="ebony homology arm"
     primer_bind     complement(6147..6163)
                     /note="M13-forward. common sequencing primer, one of
                     multiple similar variants"
     promoter        complement(6180..6198)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6205..6221)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6362..6790
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             7134..7925
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             7946..8803
                     /label=AmpR
                     /note="beta-lactamase"