pCW619-LI-dal vector (V016245)

Basic Vector Information

Vector Name:
pCW619-LI-dal
Antibiotic Resistance:
Ampicillin
Length:
8713 bp
Type:
UNVERIFIED: Cloning vector
Replication origin:
ori
Source/Author:
Yuzhen Z.

pCW619-LI-dal vector Vector Map

pCW619-LI-dal8713 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400ropbomoriAmpRAmpR promoterrRNA adenine N-6-methyltransferase

pCW619-LI-dal vector Sequence

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Download GeneBank File(.gb)

LOCUS       V016245                 8713 bp    DNA     circular SYN 20-JUN-2020
DEFINITION  Exported.
ACCESSION   V016245
VERSION     V016245
KEYWORDS    UNVERIFIED
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8713)
  AUTHORS   Yuzhen Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-2019) Department of Public Health Laboratory
            Sciences, West China School of Public HealthSichuan University, No.
            17, Section 3, Renmin South Road, Wuhou District, Chengdu, Sichuan
            610041, China
REFERENCE   2  (bases 1 to 8713)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (02-OCT-2019) Department of Public Health Laboratory Sciences, West
            China School of Public HealthSichuan University, No. 17, Section 3,
            Renmin South Road, Wuhou District, Chengdu, Sichuan 610041, China"
            GenBank staff is unable to verify sequence and/or annotation
            provided by the submitter.
FEATURES             Location/Qualifiers
     source          1..8713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             515..703
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    808..948
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1134..1722)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1896..2753)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2754..2858)
                     /label="AmpR promoter"
     CDS             6393..7124
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"

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