pcW619 vector (V016243)

Download GeneBank File(.gb)

LOCUS       V016243                 8456 bp    DNA     circular SYN 15-JUN-2020
DEFINITION  Exported.
ACCESSION   V016243
VERSION     V016243
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8456)
  AUTHORS   Zhou Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2019) Department of Public Health Laboratory
            Sciences, West China School of Public Health, Sichuan University,
            No. 17, Section 3, Renmin South Road, Wuhou District, Chengdu,
            Sichuan 610041, China
REFERENCE   2  (bases 1 to 8456)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (24-SEP-2019) Department of Public Health Laboratory Sciences, West
            China School of Public Health, Sichuan University, No. 17, Section
            3, Renmin South Road, Wuhou District, Chengdu, Sichuan 610041,
            China"
FEATURES             Location/Qualifiers
     source          1..8456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             515..703
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    808..948
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1134..1722)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1896..2753)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2754..2858)
                     /label="AmpR promoter"
     CDS             6136..6867
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"