pSSbeta vector (V016242)

LOCUS       V016242                 7446 bp    DNA     circular SYN 02-SEP-2021
DEFINITION  Exported.
ACCESSION   V016242
VERSION     V016242
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Suzuki S, Osada S, Imamura D, Sato T.
  TITLE     New Bacillus subtilis vector, pSSbeta as genetic tool for
            site-specific integration and excision of cloned DNA, and prophage
            elimination
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7446)
  AUTHORS   Suzuki S, Sato T.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2021) Contact:Shota Suzuki Department of Life
            Science, College of Science, Rikkyo University; 3-34-1
            Nishi-Ikebukuro, Toshima-ku, Tokyo 171-8501, Japan
REFERENCE   3  (bases 1 to 7446)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-AUG-2021) Contact:Shota Suzuki Department of Life Science,
            College of Science, Rikkyo University; 3-34-1 Nishi-Ikebukuro,
            Toshima-ku, Tokyo 171-8501, Japan"
FEATURES             Location/Qualifiers
     source          1..7446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            361..1998
                     /gene="sprA"
                     /label="sprA"
     CDS             361..1998
                     /codon_start=1
                     /transl_table=11
                     /gene="sprA"
                     /product="serine-type phage integrase"
                     /label="sprA"
                     /note="phage Spbeta"
                     /protein_id="BDB04359.1"
                     /translation="MELKNIVNSYNITNILGYLRRSRQDMEREKRTGEDTLTEQKELMN
                     KILTAIEIPYELKMEIGSGESIDGRPVFKECLKDLEEGKYQAIAVKEITRLSRGSYSDA
                     GQIVNLLQSKRLIIITPYKVYDPRNPVDMRQIRFELFMAREEFEMTRERMTGAKYTYAA
                     QGKWISGLAPYGYQLNKKTSKLDPVEDEAKVVQLIFNIFLNGLNGKDYSYTAIASHLTN
                     LQIPTPSGKKRWNQYTIKAILQNEVYIGTVKYKVREKTKDGKRTIRPEKEQIVVQDAHA
                     PIIDKEQFQQSQVKIANKVPLLPNKDEFELSELAGVCTCSKCGEPLSKYESKRIRKNKD
                     GTESVYHVKSLTCKKNKCTYVRYNDVENAILDYLSSLNDLNDSTLTKHINSMLSKYEDD
                     NSNMKTKKQMSEHLSQKEKELKNKENFIFDKYESGIYSDELFLKRKAALDEEFKELQNA
                     KNELNGLQDTQSEIDSNTVRNNINKIIDQYHIESSSEKKNELLRMVLKDVIVNMTQKRK
                     GPIPAQFEITPILRFNFIFDLTATNSFH"
     gene            2023..2038
                     /gene="attP"
                     /label="attP"
     misc_feature    2023..2038
                     /gene="attP"
                     /label="Bacteriophage SPbeta attachment site"
                     /note="Bacteriophage SPbeta attachment site"
     gene            complement(2186..2362)
                     /gene="sprB"
                     /label="sprB"
     CDS             complement(2186..2362)
                     /codon_start=1
                     /transl_table=11
                     /gene="sprB"
                     /product="recombination directionality factor"
                     /label="sprB"
                     /note="phage Spbeta"
                     /protein_id="BDB04360.1"
                     /translation="MEPYQRYEELKKKTIKVVQKENYSIRYITQDEASNDLDEFYKQFA
                     QHLLEAALERKAE"
     protein_bind    2442..2466
                     /label="xyl repressor encoded by xylR binding site"
                     /bound_moiety="xyl repressor encoded by xylR"
     regulatory      2476..2481
                     /label="Pxyl_-10"
                     /note="Pxyl_-10"
                     /regulatory_class="minus_10_signal"
     regulatory      2498..2504
                     /label="Pxyl_-35"
                     /note="Pxyl_-35"
                     /regulatory_class="minus_35_signal"
     gene            2610..3764
                     /gene="xylR"
                     /label="xylR"
     CDS             2610..3764
                     /codon_start=1
                     /transl_table=11
                     /gene="xylR"
                     /product="transcriptional regulator"
                     /label="xylR"
                     /protein_id="BDB04361.1"
                     /translation="MDIADQTFVKKVNQKLLLKEILKNSPISRAKLSEMTGLNKSTVSS
                     QVNTLMKESMVFEIGQGQSSGGRRPVMLVFNKKAGYSVGIDVGVDYINGILTDLEGTIV
                     LDQYRHLESNSPEITKDILIDMIHHFITQMPQSPYGFIGIGICVPGLIDKDQKIVFTPN
                     SNWRDIDLKSSIQEKYNVSVFIENEANAGAYGEKLFGAAKNHDNIIYVSISTGIGIGVI
                     INNHLYRGVSGFSGEMGHMTIDFNGPKCSCGNRGCWELYASEKALLKSLQTKEKKLSYQ
                     DIINLAHLNDIGTLNALQNFGFYLGIGLTNILNTFNPQAVILRNSIIESHPMVLNSMRS
                     EVSSRVYSQLGNSYELLPSSLGQNAPALGMSSIVIDHFLDMITM"
     CDS             complement(4065..4712)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     primer_bind     5139..5155
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    5156..5212
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(5225..5241)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5249..5265)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5273..5303)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5318..5339)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5627..6215)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6389..7246)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7247..7351)
                     /label="AmpR promoter"