pMAJIIc vector (V016238)

LOCUS       62056_16475        6684 bp DNA     circular SYN 29-JUN-2020
DEFINITION  Cloning vector pMAJIIc, complete sequence.
ACCESSION   MT119765
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Pena MM, Facincani AP, Ferreira H, Ferro JA.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2020) Departamento de Tecnologia, Univ. Estadual 
            Paulista 'Julio de Mesquita Filho' - UNESP - Jaboticabal, Via de 
            Acesso Prof.Paulo Donato Castellane s/n, Jaboticabal, SP 14884-900, 
            Brazil
REFERENCE   2  (bases 1 to 6684)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (26-FEB-2020) Departamento de Tecnologia, Univ. Estadual Paulista 
            'Julio de Mesquita Filho' - UNESP - Jaboticabal, Via de Acesso 
            Prof.Paulo Donato Castellane s/n, Jaboticabal, SP 14884-900, Brazil"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6..713
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    complement(753..1561)
                     /label=Xac_amy
                     /note="Xac_amy"
     primer_bind     complement(1577..1593)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1734..2162
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             2506..3297
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             3318..4175
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4349..4937
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5225..5246
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5261..5291
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5299..5315
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5323..5339
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(5447..6322)
                     /label=araC
                     /note="L-arabinose regulatory protein"
     promoter        6349..6633
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     regulatory      6654..6659
                     /label=ribosome_binding_site
                     /note="ribosome_binding_site"
                     /regulatory_class="ribosome_binding_site"