Basic Vector Information
- Vector Name:
- pRP1028-T1-PL
- Length:
- 8081 bp
- Type:
- Cloning vector
- Replication origin:
- pSC101 ori
- Source/Author:
- Bernhards CB, Lux MW, Katoski SE, Goralski TDP.
pRP1028-T1-PL vector Vector Map
pRP1028-T1-PL vector Sequence
LOCUS V016234 8081 bp DNA circular SYN 14-MAR-2021
DEFINITION Exported.
ACCESSION V016234
VERSION V016234
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 8081)
AUTHORS Bernhards CB, Lux MW, Katoski SE, Goralski TDP., Liem AT, Gibbons
HS.
TITLE barCoder: a tool to generate unique, orthogonal genetic tags for
qPCR detection
JOURNAL BMC Bioinformatics 22 (1), 98 (2021)
PUBMED 33648451
REFERENCE 2 (bases 1 to 8081)
AUTHORS Bernhards CB, Lux MW, Katoski SE, Goralski TDP., Liem AT, Gibbons
HS.
TITLE Direct Submission
JOURNAL Submitted (28-SEP-2020) US Army CCDC Chemical Biological Center,
8198 Blackhawk Rd, Gunpowder, MD 21010, USA
REFERENCE 3 (bases 1 to 8081)
AUTHORS .
TITLE Direct Submission
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
SGRef: number: 1; type: "Journal Article"; journalName: "BMC
Bioinformatics"; date: "2021"; volume: "22"; issue: "1"; pages: "98"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(28-SEP-2020) US Army CCDC Chemical Biological Center, 8198
Blackhawk Rd, Gunpowder, MD 21010, USA"
FEATURES Location/Qualifiers
source 1..8081
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 12..29
/label="I-SceI site"
/note="I-SceI site"
oriT 180..288
/label="oriT"
/note="incP origin of transfer"
promoter complement(303..321)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(331..347)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 909..2231
/label="ts rep from pJRS233, pWV01"
/note="ts rep from pJRS233, pWV01"
gene complement(909..1058)
/gene="orfD"
/label="orfD"
CDS complement(909..1058)
/codon_start=1
/transl_table=11
/gene="orfD"
/product="OrfD"
/label="orfD"
/protein_id="QSS48602.1"
/translation="MTNKEKELFAENEELKKEIKDLKERIERYREMEVELSTTIDLLRG
GIIE"
gene complement(1055..1753)
/gene="repA"
/label="repA"
CDS complement(1055..1753)
/codon_start=1
/transl_table=11
/gene="repA"
/product="RepA"
/label="repA"
/protein_id="QSS48609.1"
/translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
KKDKDTWNNSNIIQNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV
DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
VLDAETGEIK"
misc_difference 1578..1595
/note="mutations in repA leading to temperature
sensitivity"
gene complement(1820..1981)
/gene="orfC"
/label="orfC"
CDS complement(1820..1981)
/codon_start=1
/transl_table=11
/gene="orfC"
/product="OrfC"
/label="orfC"
/protein_id="QSS48603.1"
/translation="MVISESKKRVMISLTKEQDKKLTDMAKQKGFSKSAVAALAIEEYA
RKESEQKK"
gene complement(2022..2231)
/gene="orfB"
/label="orfB"
CDS complement(2022..2231)
/codon_start=1
/transl_table=11
/gene="orfB"
/product="OrfB"
/label="orfB"
/protein_id="QSS48604.1"
/translation="MGGKEANFASVLRPPIKCRVPIFVPKTLYPNWLKGLRGFSIANES
PTFSPTFFINLYLSSFIVVFMITK"
CDS 2365..2376
/label="Factor Xa site"
/note="Factor Xa recognition and cleavage site"
regulatory 2551..2556
/label="Ptrc"
/note="Ptrc"
/regulatory_class="minus_35_signal"
regulatory 2574..2578
/label="Ptrc"
/note="Ptrc"
/regulatory_class="minus_10_signal"
regulatory 2594..2603
/regulatory_class="ribosome_binding_site"
CDS 2611..3390
/gene="ant1"
/label="Spectinomycin 9-adenylyltransferase"
/note="Spectinomycin 9-adenylyltransferase from
Staphylococcus aureus (strain N315). Accession#: P0A0D1"
regulatory 3419..3424
/label="PrrnB"
/note="PrrnB"
/regulatory_class="minus_35_signal"
regulatory 3442..3447
/label="PrrnB"
/note="PrrnB"
/regulatory_class="minus_10_signal"
regulatory 3467..3472
/label="PFP2"
/note="PFP2"
/regulatory_class="minus_35_signal"
regulatory 3490..3495
/label="PFP2"
/note="PFP2"
/regulatory_class="minus_10_signal"
regulatory 3508..3517
/regulatory_class="ribosome_binding_site"
CDS 3544..4236
/label="TurboRFP"
/note="red fluorescent protein from Entacmaea quadricolor"
CDS complement(4767..5714)
/label="Rep101"
/note="RepA protein needed for replication with the pSC101
origin"
regulatory complement(5745..5750)
/regulatory_class="minus_10_signal"
rep_origin complement(5762..5984)
/direction=LEFT
/label="pSC101 ori"
/note="low-copy replication origin that requires the Rep101
protein"
misc_feature complement(6095..6463)
/label="par"
/note="par"
misc_feature complement(6478..8073)
/label="Btk_IntergenicTarget1_Polylinker"
/note="Btk_IntergenicTarget1_Polylinker"
misc_feature complement(join(6484..7169,7206..8069))
/note="similar to Bacillus thuringiensis serovar kurstaki
sequence in accession NZ_CP010005.1"
misc_feature 6484..6988
/note="similar to fatty acid--CoA ligase; BG08_RS29910"
misc_feature 7170..7205
/label="polylinker"
/note="polylinker"
misc_feature 7170..7175
/label="XhoI restriction site"
/note="XhoI restriction site"
misc_feature 7176..7181
/label="SmaI restriction site"
/note="SmaI restriction site"
misc_feature 7182..7187
/label="SphI restriction site"
/note="SphI restriction site"
misc_feature 7188..7193
/label="NheI restriction site"
/note="NheI restriction site"
misc_feature 7194..7199
/label="NcoI restriction site"
/note="NcoI restriction site"
misc_feature 7200..7205
/label="SacI restriction site"
/note="SacI restriction site"
CDS complement(7573..7770)
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/label="hypothetical protein"
/note="similar to BG08_RS29905"
/protein_id="QSS48608.1"
/translation="MNTTAKLINWKEHGDMIILECELNGKRFEISTYKQRIYNAHLLSD
DVYIRLDSSDNIIGINIYKK"
misc_feature complement(7905..8069)
/note="similar to hypothetical protein; BG08_RS29900"
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