Basic Vector Information
- Vector Name:
- pCG402
- Antibiotic Resistance:
- Kanamycin
- Length:
- 5269 bp
- Type:
- Cloning vector
- Replication origin:
- pBBR1 oriV
- Source/Author:
- Gao C, Fernandez VI, Lee KS, Fenizia S
pCG402 vector Map
pCG402 vector Sequence
LOCUS 62056_6260 5269 bp DNA circular SYN 02-MAY-2020 DEFINITION Cloning vector pCG402, complete sequence. ACCESSION MN744966 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5269) AUTHORS Gao C, Fernandez VI, Lee KS, Fenizia S, Pohnert G, Seymour JR, Raina J-B., Stocker R. TITLE Single-cell bacterial transcription measurements reveal the importance of dimethylsulfoniopropionate (DMSP) hotspots in ocean sulfur cycling JOURNAL Nat Commun 11 (1) (2020) In press REFERENCE 2 (bases 1 to 5269) AUTHORS Gao C. TITLE Direct Submission JOURNAL Submitted (27-NOV-2019) Biological Engineering, Massachusetts Institute of Technology, 15 Vassar Street, Cambridge, MA 20139, USA REFERENCE 3 (bases 1 to 5269) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 11 (1) (2020) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (27-NOV-2019) Biological Engineering, Massachusetts Institute of Technology, 15 Vassar Street, Cambridge, MA 20139, USA" FEATURES Location/Qualifiers source 1..5269 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin 1023..1792 /label=pBBR1 oriV /note="replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication" CDS 1793..2452 /label=pBBR1 Rep /note="replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica" regulatory 2656..2877 /label=putative PdmdA /note="putative PdmdA" /regulatory_class="promoter" CDS 2878..3573 /label=mKate2 /note="monomeric far-red fluorescent protein (Shcherbo et al., 2009)" regulatory 3598..3771 /label=TSAL2 /note="TSAL2" /regulatory_class="terminator" regulatory complement(3772..3817) /label=RNAI /note="RNAI" /regulatory_class="terminator" CDS complement(4021..4812) /label=NeoR/KanR /note="aminoglycoside phosphotransferase"
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