1-UBI_Cas9_PinII vector (V016218)

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LOCUS       62056_66        9396 bp DNA     circular SYN 19-MAR-2020
DEFINITION  Cloning vector 1-UBI_Cas9_PinII, complete sequence.
ACCESSION   MN294713
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9396)
  AUTHORS   Gao H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2019) Molecular Engineering in AST, Corteva 
            Agriscience, 8305 NW 62nd Ave., Johnston, IA 50131, USA
REFERENCE   2  (bases 1 to 9396)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (08-AUG-2019) Molecular Engineering in AST, Corteva Agriscience, 
            8305 NW 62nd Ave., Johnston, IA 50131, USA"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. 10
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      13..322
                     /regulatory_class="terminator"
     protein_bind    complement(378..477)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(495..513)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(518..534)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             647..1453
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1603..2191
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(2521..2548)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(2640..2726)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     2790..2806
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2822..2921
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3052..5046
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             5073..5093
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          5472..5660
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             6891..9389
                     /label=Cas9(C)
                     /note="C-terminal portion of Streptococcus pyogenes Cas9 
                     (Zetsche et al., 2015)"