pFLHD vector (V016214)

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LOCUS       62056_10905        9460 bp DNA     circular SYN 01-FEB-2020
DEFINITION  Cloning vector pFLHD, complete sequence.
ACCESSION   MN043927
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9460)
  AUTHORS   Gunadi A, Finer J.
  TITLE     CRISPRi validation of regulatory elements in a soybean (Glycine max)
            elongation factor-1 alpha promoter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9460)
  AUTHORS   Gunadi A, Finer J.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2019) Horticulture and Crop Science, The Ohio 
            State University, 216 Williams Hall, 1680 Madison Ave., Wooster, OH 
            44691, USA
REFERENCE   3  (bases 1 to 9460)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-JUN-2019) Horticulture and Crop Science, The Ohio State 
            University, 216 Williams Hall, 1680 Madison Ave., Wooster, OH 44691,
            USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..253
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(295..311)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(319..335)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(343..373)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(388..409)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(697..1285)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1459..2316)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2317..2421)
                     /label=AmpR promoter
     regulatory      2745..5169
                     /label=GmubiXL promoter from soybean
                     /note="GmubiXL promoter from soybean"
                     /regulatory_class="promoter"
     CDS             5303..9403
                     /label=dCas9
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Streptococcus pyogenes Type II CRISPR/Cas system"