pgRNA-ccdB-2 vector (V016211)

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LOCUS       62056_11895        5757 bp DNA     circular SYN 09-AUG-2020
DEFINITION  Cloning vector pgRNA-ccdB-2, complete sequence.
ACCESSION   MN934324
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Zhang K, Zhang Z, Kang J, Chen J, Liu J, Gao N, Fan L, Zheng P, Wang
            Y, Sun J.
  TITLE     CRISPR/Cas13d-Mediated Microbial RNA Knockdown
  JOURNAL   Front Bioeng Biotechnol 8, 856 (2020)
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Wang Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2020) Key Laboratory of Systems Microbial 
            Biotechnology, Tianjin Institute of Industrial Biotechnology, 
            Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport 
            Economic Area, Tianjin 300308, China
REFERENCE   3  (bases 1 to 5757)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol 8, 856 (2020)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-JAN-2020) Key Laboratory of Systems Microbial Biotechnology, 
            Tianjin Institute of Industrial Biotechnology, Chinese Academy of 
            Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin
            300308, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..53
                     /regulatory_class="promoter"
     misc_RNA        54..83
                     /label=RfxCas13d DR30
                     /note="5' processed 30-base direct repeat for an RfxCas13d
                     gRNA (Konermann et al., 2018)"
     CDS             432..734
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     terminator      753..839
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      931..958
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     rep_origin      complement(2055..3518)
                     /direction=LEFT
     CDS             4042..4833
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      4936..5524
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"