pR6K-KCA vector (V016201)

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LOCUS       62056_18715        2578 bp DNA     circular SYN 06-JUN-2019
DEFINITION  Cloning vector pR6K-KCA, complete sequence.
ACCESSION   MK787297
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Moore CL, Papa LJ III, Shoulders MD.
  TITLE     A Processive Protein Chimera Introduces Mutations across Defined DNA
            Regions In Vivo
  JOURNAL   J. Am. Chem. Soc. 140 (37), 11560-11564 (2018)
  PUBMED    29991261
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Moore CL, Papa LJ III, Shoulders MD.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2019) Chemistry, Massachusetts Institute of 
            Technology, 77 Massachusetts Avenue, 16-573A, Cambridge, MA 02139, 
            USA
REFERENCE   3  (bases 1 to 2578)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Am. 
            Chem. Soc."; date: "2018"; volume: "140"; issue: "37"; pages: 
            "11560-11564"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-APR-2019) Chemistry, Massachusetts Institute of Technology, 77 
            Massachusetts Avenue, 16-573A, Cambridge, MA 02139, USA"
FEATURES             Location/Qualifiers
     source          1..2578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             23..325
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     promoter        374..404
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     regulatory      440..451
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
                     /regulatory_class="ribosome_binding_site"
     CDS             458..1120
                     /label=amilCP
                     /note="amilCP is a fluorescent protein published in 2008, 
                     derived from Acropora millepora. It is reported to be a 
                     somewhat slowly-maturing tetramer."
     rep_origin      1180..1568
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     promoter        1652..1699
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             1718..2518
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"