pMK201.2 vector (V016173)

MSDS

Basic Vector Information

Vector Name:: pMK201.2

Antibiotic Resistance:: Chloramphenicol

Length:: 3848 bp

Type:: Cloning vector

Replication origin:: ori

Source/Author:: Hartsough LA, Kotlajich MV, Han B, Lin C-C.J.

pMK201.2 vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMK201.2 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V016173                 3848 bp    DNA     circular SYN 27-JUL-2020
DEFINITION  Exported.
ACCESSION   V016173
VERSION     V016173
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Hartsough LA, Kotlajich MV, Han B, Lin C-C.J., Gambill L, Wang MC,
            Tabor JJ.
  TITLE     Optogenetic control of gut bacterial metabolism
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Hartsough LA, Kotlajich MV, Han B, Lin C-C.J., Gambill L, Wang MC,
            Tabor JJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2019) Bioengineering, Rice University, 6500 Main
            St., Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 3848)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-OCT-2019) Bioengineering, Rice University, 6500 Main St.,
            Houston, TX 77030, USA"
FEATURES             Location/Qualifiers
     source          1..3848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..35
                     /label="constitutive promoter J23100"
                     /note="constitutive promoter J23100"
     misc_feature    36..69
                     /label="B0034"
                     /note="B0034"
     misc_feature    70..774
                     /label="ccaR"
                     /note="ccaR"
     terminator      795..866
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      882..909
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    916..1020
                     /label="pEXT20 expression vector"
                     /note="pEXT20 expression vector"
     misc_feature    1033..1204
                     /label="PcpcG2-172"
                     /note="PcpcG2-172"
     misc_feature    1137..1158
                     /label="G box"
                     /note="G box"
     misc_feature    1205..1224
                     /label="B0034"
                     /note="B0034"
     misc_feature    1225..1230
                     /label="biobricks suffix"
                     /note="biobricks suffix"
     CDS             1231..1851
                     /gene="rcsA"
                     /label="Transcriptional regulatory protein RcsA"
                     /note="Transcriptional regulatory protein RcsA from
                     Escherichia coli (strain K12). Accession#: P0DMC9"
     terminator      complement(1878..1921)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2191..2779)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(2867..2961)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(2985..3641)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"

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