pMK201.2 vector (V016173)

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LOCUS       V016173                 3848 bp    DNA     circular SYN 27-JUL-2020
DEFINITION  Exported.
ACCESSION   V016173
VERSION     V016173
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Hartsough LA, Kotlajich MV, Han B, Lin C-C.J., Gambill L, Wang MC,
            Tabor JJ.
  TITLE     Optogenetic control of gut bacterial metabolism
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Hartsough LA, Kotlajich MV, Han B, Lin C-C.J., Gambill L, Wang MC,
            Tabor JJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2019) Bioengineering, Rice University, 6500 Main
            St., Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 3848)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-OCT-2019) Bioengineering, Rice University, 6500 Main St.,
            Houston, TX 77030, USA"
FEATURES             Location/Qualifiers
     source          1..3848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..35
                     /label="constitutive promoter J23100"
                     /note="constitutive promoter J23100"
     misc_feature    36..69
                     /label="B0034"
                     /note="B0034"
     misc_feature    70..774
                     /label="ccaR"
                     /note="ccaR"
     terminator      795..866
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      882..909
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    916..1020
                     /label="pEXT20 expression vector"
                     /note="pEXT20 expression vector"
     misc_feature    1033..1204
                     /label="PcpcG2-172"
                     /note="PcpcG2-172"
     misc_feature    1137..1158
                     /label="G box"
                     /note="G box"
     misc_feature    1205..1224
                     /label="B0034"
                     /note="B0034"
     misc_feature    1225..1230
                     /label="biobricks suffix"
                     /note="biobricks suffix"
     CDS             1231..1851
                     /gene="rcsA"
                     /label="Transcriptional regulatory protein RcsA"
                     /note="Transcriptional regulatory protein RcsA from
                     Escherichia coli (strain K12). Accession#: P0DMC9"
     terminator      complement(1878..1921)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2191..2779)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(2867..2961)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(2985..3641)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"