pT7-NSP1(02V0002G3) vector (V016169)

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LOCUS       62056_20775        4540 bp DNA     circular SYN 15-DEC-2020
DEFINITION  Cloning vector pT7-NSP1(02V0002G3), complete sequence.
ACCESSION   MN689785
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Potential of avian and mammalian species A rotaviruses to reassort 
            as explored by plasmid only-based reverse genetics
  JOURNAL   Virus Res 286, 198027 (2020)
  PUBMED    32442596
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2019) Unit Viruses in Food, German Federal 
            Institute for Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, 
            Germany
REFERENCE   3  (bases 1 to 4540)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virus Res 
            286, 198027 (2020)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2019) Unit Viruses in Food, German Federal Institute for 
            Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, Germany"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        233..251
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           250..287
     gene            288..2021
                     /gene="NSP1"
                     /label=NSP1
     CDS             288..2021
                     /codon_start=1
                     /transl_table=11
                     /gene="NSP1"
                     /product="NSP1"
                     /label=NSP1
                     /note="derived from Rotavirus A strain 02V0002G3"
                     /protein_id="QPP12454.1"
                     /translation="MASNQLRFEYSVAKFTRSCFTPTLKSTEYVNSMQWLSKERLNRHL
                     WRTFNRYMIDDLPKGNCWTCGIFHNVYSCDFCNVNHICRSCKENRIDLCPFVSHTTCRF
                     AHDLIRIENCEKLTDDVLLHNLIPLYEKYFKILSKISRSAVWKELSEKKRRGQRMHTTS
                     SVAVDFNDCFLPTNIIAFKSVDKCNVIGYEKIYMNLLFGHYEPQRNREQMISYTNINLK
                     QFRKTFDLITNMNLQLALSRGKALNSMVNMDVSSNVVEIPYVNDQSYICQLDDSKLNYA
                     LIHFDAPEKFTLCRPILSCFHPEQLLKEIFTSWMVINVRVALTKLERSHYDSIFNLTNQ
                     ILWPNQERNYTSWLYFSYYENLVHDVLDNLQDIRKFAGASVHAARHVGLHESDCISCRK
                     YNYFIDVEMARKVSKILMKTCCYHDTRCADITTIFSISHRRNHDELVWRTFFDLRATLI
                     HNANIVRNLLNQSGLIAEFDTLCEWSDSSKLKLRHFCAKDNTEKLDLLQKFWSLSSDVR
                     DKMVKKFNINTDYYKVFEILPRDSEVLISITTEYDYELVTRSNSFKWLANILEIERIDD
                     E"
     3'UTR           2022..2371
     terminator      2532..2579
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(2748..2764)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      2812..2843
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        2844..2946
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             2947..3804
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      3879..3906
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(3918..4505)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"