pJL74-2w-gpmcherry-cat vector (V016150)

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LOCUS       V016150                 6760 bp    DNA     circular SYN 11-DEC-2019
DEFINITION  Exported.
ACCESSION   V016150
VERSION     V016150
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Leech JM, Dhariwala MO, Lowe MM, Chu K, Merana GR, Cornuot C, Weckel
            A, Ma JM, Leitner EG, Gonzalez JR, Vasquez KS, Diep BA, Scharschmidt
            TC.
  TITLE     Toxin-Triggered Interleukin-1 Receptor Signaling Enables Early-Life
            Discrimination of Pathogenic versus Commensal Skin Bacteria
  JOURNAL   Cell Host Microbe (2019) In press
   PUBMED   31784259
REFERENCE   2  (bases 1 to 6760)
  AUTHORS   Scharschmidt TC.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2019) Dermatology, University of California San
            Francisco, 513 Parnassus Ave, San Francisco, CA 94143, USA
REFERENCE   3  (bases 1 to 6760)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host
            Microbe (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-MAY-2019) Dermatology, University of California San Francisco,
            513 Parnassus Ave, San Francisco, CA 94143, USA"
FEATURES             Location/Qualifiers
     source          1..6760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..251
                     /regulatory_class="promoter"
     CDS             342..1046
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     regulatory      1058..1357
                     /regulatory_class="terminator"
     rep_origin      1364..3448
     CDS             3653..4300
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     promoter        4906..5010
                     /label="AmpR promoter"
     CDS             5011..5868
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6042..6630
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"