Basic Vector Information
- Vector Name:
- pMJA284
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6739 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Pscheid R, Wang R, Allegrucci C, Alcocer M.
pMJA284 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMJA284 vector Sequence
LOCUS V016126 6739 bp DNA circular SYN 02-FEB-2019
DEFINITION Exported.
ACCESSION V016126
VERSION V016126
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 6739)
AUTHORS Pscheid R, Wang R, Allegrucci C, Alcocer M.
TITLE The intrinsic allergenicity of nut proteins: a lipid story
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6739)
AUTHORS Pscheid R, Wang R, Allegrucci C, Alcocer M.
TITLE Direct Submission
JOURNAL Submitted (22-AUG-2018) School of Biosciences, University of
Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire
LE12 5RD, United Kingdom
REFERENCE 3 (bases 1 to 6739)
AUTHORS .
TITLE Direct Submission
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(22-AUG-2018) School of Biosciences, University of Nottingham,
Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD,
United Kingdom"
FEATURES Location/Qualifiers
source 1..6739
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR 117..750
/label="3' LTR"
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 797..922
/label="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1415..1648
/label="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1833..1877
/label="gp41 peptide"
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 2026..2067
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
/label="Protein Tat"
misc_feature 2164..2281
/label="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2428..2639
/label="EF-1-alpha core promoter"
/note="core promoter for human elongation factor
EF-1-alpha"
misc_feature 2721..2779
/label="MCS"
/note="MCS"
misc_feature 2855..3441
/label="IRES2"
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 3461..4057
/label="PuroR"
/note="puromycin N-acetyltransferase"
LTR 4263..4496
/label="3' LTR (Delta-U3)"
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 4653..4877
/label="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
promoter 4973..5077
/label="AmpR promoter"
CDS 5078..5935
/label="AmpR"
/note="beta-lactamase"
rep_origin 6109..6697
/direction=RIGHT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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