pTrichoGate-20 vector (V016120)

LOCUS       62056_21510        4534 bp DNA     circular SYN 28-AUG-2019
DEFINITION  Cloning vector pTrichoGate-20, complete sequence.
ACCESSION   MN007124
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Nogueira-Lopez G.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd, 
            Lincoln, Christchurch, Canterbury 7647, New Zealand
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd, Lincoln, 
            Christchurch, Canterbury 7647, New Zealand"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(35..602)
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus
                     nidulans trpC gene"
     CDS             complement(760..1551)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(1552..1836)
                     /label=tryptophan synthase promoter (PtrpC)
                     /note="tryptophan synthase promoter (PtrpC)"
                     /regulatory_class="promoter"
     primer_bind     complement(1899..1915)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1923..1939)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1947..1977)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1992..2013)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2301..2889)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3063..3920)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3921..4025)
                     /label=AmpR promoter
     primer_bind     4499..4515
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"