p57-V5+NeoR vector (V016116)

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LOCUS       62056_2200        4695 bp DNA     circular SYN 25-SEP-2019
DEFINITION  Cloning vector p57-V5+NeoR, complete sequence.
ACCESSION   MN047315
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Kachale A, Kraeva N, Bianchi C, Yurchenko V, Lukes J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2019) Laboratory of Molecular Biology of Protists,
            Biology Centre CAS, Institute of Parasitology, Branisovska 31, Ceske
            Budejovice, Sb 37005, Czech Republic
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (07-JUN-2019) Laboratory of Molecular Biology of Protists, Biology 
            Centre CAS, Institute of Parasitology, Branisovska 31, Ceske 
            Budejovice, Sb 37005, Czech Republic"
FEATURES             Location/Qualifiers
     source          1..4695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           663..1082
                     /note="5' noncoding region of hexokinase gene (420bp part)
                     from Blastocrithidia sp. p57"
     CDS             1086..1127
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             1221..2018
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     3'UTR           2022..2441
                     /note="3' noncoding region of hexokinase gene (420bp part)
                     from Blastocrithidia sp. p57"
     primer_bind     complement(2454..2470)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2507..2525)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2546..2562)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2570..2586)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2594..2624)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2639..2660)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2948..3536)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3710..4567)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4568..4672)
                     /label=AmpR promoter