AGG1186-pHr5Fluc vector (V016111)

MSDS

Basic Vector Information

Vector Name:: AGG1186-pHr5Fluc

Antibiotic Resistance:: Ampicillin

Length:: 6112 bp

Type:: Cloning vector

Replication origin:: ori

Source/Author:: Tng PYL., Carabajal Paladino L, Verkuijl SAN., Purcell J

Promoter:: IE1

AGG1186-pHr5Fluc vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

AGG1186-pHr5Fluc vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_291        6112 bp DNA     circular SYN 12-AUG-2020
DEFINITION  Cloning vector AGG1186:pHr5Fluc, complete sequence.
ACCESSION   MT119956
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Tng PYL., Carabajal Paladino L, Verkuijl SAN., Purcell J, Merits A, 
            Leftwich PT, Fragkoudis R, Noad R, Alphey L.
  TITLE     Cas13b-dependent and Cas13b-independent RNA knockdown of viral 
            sequences in mosquito cells following guide RNA expression
  JOURNAL   Commun Biol 3 (1), 413 (2020)
  PUBMED    32737398
REFERENCE   2  (bases 1 to 6112)
  AUTHORS   Tng PYL., Carabajal Paladino L, Verkuijl S, Purcell J, Merits A, 
            Leftwich PT, Fragkoudis R, Noad R, Alphey L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2020) Arthropod Genetics Group, The Pirbright 
            Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK
REFERENCE   3  (bases 1 to 6112)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol"; date: "2020"; volume: "3"; issue: "1"; pages: "413"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-FEB-2020) Arthropod Genetics Group, The Pirbright Institute, Ash
            Road, Pirbright, Woking, Surrey GU24 0NF, UK"
FEATURES             Location/Qualifiers
     source          1..6112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        134..616
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     intron          1360..1429
                     /note="adh"
     misc_feature    1432..1440
                     /label=Kozak sequence
                     /note="Kozak sequence"
     CDS             1441..3090
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(3134..3255)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3674..4262)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4436..5293)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5294..5398)
                     /label=AmpR promoter
     rep_origin      5425..5880
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    6011..6059
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    6073..6112
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"

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