pCJH6_pETcc2--CaNOX vector (V016109)

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LOCUS       62056_6475        3396 bp DNA     circular SYN 12-DEC-2019
DEFINITION  Cloning vector pCJH6_pETcc2::CaNOX, complete sequence.
ACCESSION   MK910754
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG,
            Nebl T, Coia G, Warden AC, Simpson G, Frazer AR, Nixon Jensen C, 
            Turner NJ, Scott C.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG,
            Nebl T, Coia G, Warden AC, Simpson G, Frazer AR, Nixon Jensen C, 
            Turner NJ, Scott C.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 3396)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (10-MAY-2019) Biocatalysis "
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-MAY-2019) Biocatalysis "
FEATURES             Location/Qualifiers
     source          1..3396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..1350
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /EC_number="1.6.3.4"
                     /label=NADH oxidase
                     /note="from Clostridium aminoverlaricum (BAE53714)"
                     /protein_id="QGT40742.1"
                     /translation="MKIVVIGCTHAGTAAVKTILKENPEAEITIFERNDNISFLSCGIA
                     LYVGGVVKDPAGLFYSNPEELSKMGANVKIKHNVKSIDTKSKKVIAEDMNTGEEIEVSY
                     DKLVNTTGSWPIIPPIPGIESKNILLCKNYDQANVIIRQTKDAKKIVIVGGGYIGIELV
                     EAFQKSGKQVTLIDGLDRILNKYLDKEFTDILEDDLKKNGINLALDQCVKSFKANENGE
                     VTSVETTKGEYEADMVILCVGFRPNNELLKGKVDMLPNDAIIVDEYMRTSDPDIFAAGD
                     SCAVHYNPNGNYAYIPLATNAVRMGMLIGKNISTPKVKYRGTQSTSGLNLFGYNIGSTG
                     VTVSGAPQIGLNVRSVIVKDNYRPEFMPTNEEIIMQLVYEVGTNRIVGGQVMSKYDITQ
                     SANTLSLAIQNKMTIEDLAYVDFFFQPHFDRPWNYLNILGLAALEQEGL"
     promoter        1457..1561
                     /label=AmpR promoter
     CDS             1562..2419
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2593..3181
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3261..3279
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             3311..3333
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3352..3369
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             3379..3396
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"