pNATX13-hmtC vector (V016104)

LOCUS       62056_17720        6033 bp RNA     circular SYN 30-AUG-2020
DEFINITION  Shuttle vector pNATX13-hmtC, complete sequence.
ACCESSION   MT881545
VERSION     .
KEYWORDS    .
SOURCE      
  ORGANISM  
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Chi Z, Chi Z, Kong C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2020) Ocean University of China, Qing Dao, 
            Shandong 266000, China
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (30-JUL-2020) Ocean University of China, Qing Dao, Shandong 266000, 
            China"
FEATURES             Location/Qualifiers
     source          1..6033
                     /mol_type="other DNA"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            3101..3745
                     /gene="hmtC"
                     /label=hmtC
     CDS             3101..3745
                     /codon_start=1
                     /transl_table=11
                     /gene="hmtC"
                     /product="piperazate synthase"
                     /label=hmtC
                     /protein_id="QNJ99249.1"
                     /translation="MFVPSHYREPDSSWMVDIIRGNPLALMMSNGAAGEPPFATHLPVI
                     PDPAMTGDWSERLSEATLLGHMNRDNPQWQALEDGAVVRIAFSGPHAYVSPTLYGVTPA
                     APTWNFTSVHVRGVVERIPSTEETLEVVKSTVRAFEADFGEGWDMAASIDYFRKIVPGV
                     GAFRIMVRNVDGMFKLSQEQQPEVRDRVRKSFAGRECGRHQETAAYMSRLP"
     protein_bind    complement(3752..3785)
                     /label=lox71
                     /note="Left element (LE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    complement(3796..3970)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4014..4580)
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     protein_bind    complement(5027..5060)
                     /label=lox66
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5639..5655)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"