pNM261 vector (V016095)

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LOCUS       62056_18090        8395 bp DNA     circular SYN 16-DEC-2019
DEFINITION  Cloning vector pNM261, complete sequence.
ACCESSION   MN443784
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Gonzalez LM, Mukhitov N, Voigt CA.
  TITLE     Resilient living materials built by printing bacterial spores
  JOURNAL   Nat. Chem. Biol. (2019) In press
  PUBMED    31792444
REFERENCE   2  (bases 1 to 8395)
  AUTHORS   Mukhitov N, Gonzalez LM, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2019) Biological Engineering, MIT, NE47-140, 500 
            Tech Square, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 8395)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem. 
            Biol. (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-SEP-2019) Biological Engineering, MIT, NE47-140, 500 Tech 
            Square, Cambridge, MA 02139, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        106..210
                     /label=AmpR promoter
     CDS             211..1068
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2963..4138
                     /label=similar to AmyE
                     /note="similar to AmyE"
     gene            complement(4263..5045)
                     /gene="specR"
                     /label=specR
     terminator      5341..5427
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      5446..5473
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     misc_binding    5593..5604
                     /label=VanO binding site
                     /bound_moiety="VanO"
     regulatory      5618..5647
                     /label=Pspank
                     /note="Pspank"
                     /regulatory_class="promoter"
     misc_binding    5647..5658
                     /label=VanO binding site
                     /bound_moiety="VanO"
     misc_RNA        5660..5710
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     regulatory      5741..5757
                     /label=gsiB
                     /note="gsiB"
                     /regulatory_class="ribosome_binding_site"
     CDS             5758..6495
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin"
                     /label=lysostaphin
                     /protein_id="QGV56745.1"
                     /translation="MTHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVK
                     AISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGS
                     TGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTGWKTNKYGTLY
                     KSESASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSGQR
                     IYLPVRTWNKSTNTLGVLWGTIK"
     terminator      6531..6625
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     regulatory      6733..6803
                     /label=Ppen
                     /note="Ppen"
                     /regulatory_class="promoter"
     regulatory      6811..6827
                     /label=spoVG
                     /note="spoVG"
                     /regulatory_class="ribosome_binding_site"
     CDS             6828..7562
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-IVS"
                     /label=VanR-IVS
                     /protein_id="QGV56746.1"
                     /translation="MDMPRIKPGQRVMMALRKMIASGEIKSGERIAEIPTAAALGVSRM
                     PVRIALRSLEQEGLVVRLGARGYAARGVSSDQIRDAIEVRGVLEGFAARRLAERGMTAE
                     THARFVVLIAEGEALFAAGRLNGEDLDRYAAYNQAFHDTLVSAAGNGAVESALARNGFE
                     PFAAAGALALDLMDLSAEYEHLLAAHRQHQAVLDAVSCGDAEGAERIMRDHALAAIRNA
                     KVFEAAASAGAPLGAAWSIRAD"
     regulatory      7686..7706
                     /label=hairpin terminator
                     /note="hairpin terminator"
                     /regulatory_class="terminator"
     misc_feature    7728..8395
                     /label=similar to AmyE
                     /note="similar to AmyE"