pMJA219 vector (V016091)

MSDS

Basic Vector Information

Vector Name:: pMJA219

Antibiotic Resistance:: Ampicillin

Length:: 8065 bp

Type:: Cloning vector

Replication origin:: ori

Source/Author:: Wang R, Pscheid R, Ghumra A, Alcocer M.

Promoter:: CMV

pMJA219 vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMJA219 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V016091                 8065 bp    DNA     circular SYN 02-FEB-2019
DEFINITION  Exported.
ACCESSION   V016091
VERSION     V016091
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Wang R, Pscheid R, Ghumra A, Alcocer M.
  TITLE     The intrinsic allergenicity of nut proteins: a lipid story
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8065)
  AUTHORS   Wang R, Pscheid R, Ghumra A, Alcocer M.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2018) School of Biosciences, University of
            Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire
            LE12 5RD, United Kingdom
REFERENCE   3  (bases 1 to 8065)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-AUG-2018) School of Biosciences, University of Nottingham,
            Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD,
            United Kingdom"
FEATURES             Location/Qualifiers
     source          1..8065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(570..625)
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     CDS             complement(782..1726)
                     /gene="TRB"
                     /label="T cell receptor beta chain MC.7.G5"
                     /note="T cell receptor beta chain MC.7.G5 from Homo
                     sapiens. Accession#: P0DTU4"
     promoter        complement(1892..2095)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(2096..2399)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             2714..3055
                     /gene="TRAV10"
                     /label="T cell receptor alpha variable 10"
                     /note="T cell receptor alpha variable 10 from Homo sapiens.
                     Accession#: A0A0B4J240"
     CDS             3122..3541
                     /gene="TRAC"
                     /label="T cell receptor alpha chain constant"
                     /note="T cell receptor alpha chain constant from Homo
                     sapiens. Accession#: P01848"
     CDS             3563..3604
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             3614..3631
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    3660..3884
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3930..4358
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4372..4701
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4768..5559
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5738..5871
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5908..5924)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5932..5948)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5956..5986)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6001..6022)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6310..6898)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7072..7929)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7930..8034)
                     /label="AmpR promoter"

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