pMU1817 vector (V016083)

LOCUS       V016083                 9876 bp    DNA     circular SYN 28-JAN-2019
DEFINITION  Exported.
ACCESSION   V016083
VERSION     V016083
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9876)
  AUTHORS   Holwerda EK, Olson DG, Ruppertsburger NM, Stevenson D, Murphy SJL.,
            Maloney MI, Lanahan A, Amador- Noguez D, Lynd LR.
  TITLE     Evolutionary response of Clostridium thermocellum to genetic
            modifications aimed at improving ethanol production
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9876)
  AUTHORS   Olson DG, Argyros DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2018) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 9876)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-OCT-2018) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
FEATURES             Location/Qualifiers
     source          1..9876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(294..1094)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1095..1315)
                     /label="URA3 promoter"
     misc_feature    1343..1846
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     misc_feature    complement(1888..2922)
                     /label="3' homology region"
                     /note="3' homology region"
     misc_feature    complement(2923..3765)
                     /label="5' homology region"
                     /note="5' homology region"
     gene            complement(3884..4429)
                     /gene="hpt"
                     /label="hpt"
     CDS             complement(3884..4429)
                     /codon_start=1
                     /transl_table=11
                     /gene="hpt"
                     /product="hypoxanthine phosphoribosyltransferase"
                     /label="hpt"
                     /protein_id="QAT79155.1"
                     /translation="MENLSKDIDEILITEEELKEKIKELGRQITKDYKGKNLMLVGVLK
                     GALMFMADLSRHIDLPLSLDFMAVSSYGSSTHSSGIVKIIKDLDISIEGKDVLIVEDII
                     DSGLTLSYLRETLLGRKPKSLKICTILDKPERREASVKVDYVGFKIPDKFVVGYGLDFD
                     EKYRNLPFIGVLKPEMYS"
     CDS             complement(4455..5102)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     regulatory      complement(5103..5630)
                     /label="gapDH promoter from Clostridium thermocellum"
                     /note="gapDH promoter from Clostridium thermocellum"
                     /regulatory_class="promoter"
     misc_feature    complement(5631..6262)
                     /label="internal homology region"
                     /note="internal homology region"
     gene            complement(6263..6841)
                     /gene="tdk"
                     /label="tdk"
     CDS             complement(6263..6841)
                     /codon_start=1
                     /transl_table=11
                     /gene="tdk"
                     /product="thymidine dikinase"
                     /label="tdk"
                     /protein_id="QAT79157.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     regulatory      complement(6842..7462)
                     /label="Clostridium thermocellum cbp promoter"
                     /note="Clostridium thermocellum cbp promoter"
                     /regulatory_class="promoter"
     CDS             complement(7572..8573)
                     /label="repB"
                     /note="RepB replication protein"
     rep_origin      complement(9265..9853)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"