Basic Vector Information
- Vector Name:
- pNC-BiFC-ED2
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 9874 bp
- Type:
- Plant binary expression vector
- Replication origin:
- pSa ori
- Host:
- Plants
- Source/Author:
- Yan P, Zeng Y, Shen W, Tuo D
- Promoter:
- CaMV 35S (enhanced)
pNC-BiFC-ED2 vector Vector Map
pNC-BiFC-ED2 vector Sequence
LOCUS 62056_17765 9874 bp DNA circular SYN 04-SEP-2019
DEFINITION Plant binary expression vector pNC-BiFC-ED2, complete sequence.
ACCESSION MK896891
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9874)
AUTHORS Yan P, Zeng Y, Shen W, Tuo D, Li X, Zhou P.
TITLE Direct Submission
JOURNAL Submitted (07-MAY-2019) Institute of Tropical Bioscience and
Biotechnology, Chinese Academy of Tropical Agriculture Sciences,
Xueyuan Road, Haikou, Hainan 571101, China
REFERENCE 2 (bases 1 to 9874)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(07-MAY-2019) Institute of Tropical Bioscience and Biotechnology,
Chinese Academy of Tropical Agriculture Sciences, Xueyuan Road,
Haikou, Hainan 571101, China"
FEATURES Location/Qualifiers
source 1..9874
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 10..26
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 118..789
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
regulatory 799..929
/label=translational enhancer
/note="translational enhancer"
/regulatory_class="enhancer"
CDS 932..1450
/label=VN173
/note="N-terminal fragment of mVenus for use in bimolecular
fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
promoter 1650..1752
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 1783..2085
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
regulatory 2155..2365
/label=CaMV 35S terminator
/note="CaMV 35S terminator"
/regulatory_class="terminator"
CDS complement(2475..3128)
/label=CmR
/note="chloramphenicol acetyltransferase"
regulatory 3227..3437
/label=CaMV 35S terminator
/note="CaMV 35S terminator"
/regulatory_class="terminator"
gene 3459..3653
/gene="CEYFP"
/label=CEYFP
CDS complement(3714..4016)
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
promoter complement(4047..4149)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
regulatory 4332..4462
/label=translational enhancer
/note="translational enhancer"
/regulatory_class="enhancer"
promoter complement(4472..5143)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
primer_bind complement(5235..5251)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5274..5290)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(5327..5345)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(5366..5382)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(5390..5406)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5414..5445)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(5460..5481)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 5720..5744
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin complement(5835..6423)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6527..7339)
/label=KanR
/note="aminoglycoside phosphotransferase"
rep_origin 7630..8065
/label=pSa ori
/note="origin of replication from bacterial plasmid pSa"
misc_feature 8198..8220
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA
(truncated)"
terminator complement(8244..8495)
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(8538..9329)
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
promoter complement(9363..9542)
/label=NOS promoter
/note="nopaline synthase promoter"
promoter 9812..9830
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 9856..9872
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
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