pDRE vector (V016062)

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LOCUS       V016062                 5329 bp    DNA     circular SYN 10-FEB-2019
DEFINITION  Exported.
ACCESSION   V016062
VERSION     V016062
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Hua M.
  TITLE     A dual-replicon shuttle vector system for heterologous replication
            and expression in a broad range of gram-positive and negative
            bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   Hua M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JAN-2018) Institute of Infectious Diseases, Beijing
            Ditan Hospital, No. 8 Jingshundongjie, Beijing 100015, China,
            Beijing, Beijing 100015, China
REFERENCE   3  (bases 1 to 5329)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-JAN-2018) Institute of Infectious Diseases, Beijing Ditan
            Hospital, No. 8 Jingshundongjie, Beijing 100015, China, Beijing,
            Beijing 100015, China"
FEATURES             Location/Qualifiers
     source          1..5329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      49..80
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        81..183
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             184..1041
                     /label="AmpR"
                     /note="beta-lactamase"
     terminator      1068..1095
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1107..1694)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(1716..1745)
                     /label="T3Te terminator"
                     /note="phage T3 early transcription terminator"
     CDS             3181..3315
                     /gene="copG"
                     /label="Protein CopG"
                     /note="Protein CopG from Streptococcus agalactiae.
                     Accession#: P13920"
     CDS             3379..4011
                     /codon_start=1
                     /transl_table=11
                     /product="RepB"
                     /label="RepB"
                     /protein_id="QBA30996.1"
                     /translation="MAKEKARYFTFLLYPESIPSDWELKLETLGVPMAISPLHDKDKSS
                     IKGQKYKKAHYHVLYIAKNPVTADSVRKKIKLLLGEKSLAMVQVVLNVENMYLYLTHES
                     KDAIAKKKHVYDKADIKLINNFDIDRYVTLDVEEKTELFNVVVSLIRAYTLQNIFDLYD
                     FIDENGETYGLTINLVNEVIAGKTGFMKLLFDGAYQRSKRGTKNEER"
     CDS             4262..5245
                     /gene="malR"
                     /label="HTH-type transcriptional regulator MalR"
                     /note="HTH-type transcriptional regulator MalR from
                     Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 /
                     TIGR4). Accession#: P0A4T1"