pTR-CB-GFP vector (V016054)

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LOCUS       62056_21345        5763 bp DNA     circular SYN 26-JUN-2019
DEFINITION  Recombinant vector pTR-CB-GFP, complete sequence.
ACCESSION   MK225672
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   O'Connor DM, Lutomski C, Jarrold MF, Boulis NM, Donsante A.
  TITLE     Lot-to-lot Variation in Adeno-associated Virus Serotype 9 (AAV9) 
            Preparations
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   O'Connor DM, Lutomski C, Jarrold MF, Boulis NM, Donsante A.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2018) Neurosurgery, Emory University, 101 Woodruff
            Circle, Room 6339, Atlanta, GA 30322, USA
REFERENCE   3  (bases 1 to 5763)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-NOV-2018) Neurosurgery, Emory University, 101 Woodruff Circle, 
            Room 6339, Atlanta, GA 30322, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    44..251
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   264..404
     misc_feature    complement(426..920)
                     /label=similar to pGFIB-1 in INSD accession LC121522.1
                     /note="similar to pGFIB-1 in INSD accession LC121522.1"
     promoter        1202..1306
                     /label=AmpR promoter
     CDS             1307..2164
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2338..2926
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      3533..4046
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     repeat_region   4090..4195
                     /label=mutant
                     /note="mutant"
     regulatory      4242..4521
                     /label=CMV enhancer
                     /note="CMV enhancer"
                     /regulatory_class="enhancer"
     regulatory      4528..4797
                     /label=derived from chicken B-actin
                     /note="derived from chicken B-actin"
                     /regulatory_class="promoter"
     intron          4863..4959
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             5033..5749
                     /label=EGFP
                     /note="enhanced GFP"