Basic Vector Information
- Vector Name:
- pNC-BiFC-Enn
- Antibiotic Resistance:
- Kanamycin
- Length:
- 6987 bp
- Type:
- Plant binary expression vector
- Replication origin:
- pSa ori
- Host:
- Plants
- Source/Author:
- Yan P, Zeng Y, Shen W, Tuo D
- Promoter:
- CaMV 35S (enhanced)
pNC-BiFC-Enn vector Map
pNC-BiFC-Enn vector Sequence
LOCUS 62056_17785 6987 bp DNA circular SYN 04-SEP-2019 DEFINITION Plant binary expression vector pNC-BiFC-Enn, complete sequence. ACCESSION MK896895 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6987) AUTHORS Yan P, Zeng Y, Shen W, Tuo D, Li X, Zhou P. TITLE Direct Submission JOURNAL Submitted (07-MAY-2019) Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Xueyuan Road, Haikou, Hainan 571101, China REFERENCE 2 (bases 1 to 6987) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (07-MAY-2019) Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Xueyuan Road, Haikou, Hainan 571101, China" FEATURES Location/Qualifiers source 1..6987 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 10..26 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 118..789 /label=CaMV 35S promoter (enhanced) /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" regulatory 799..929 /label=translational enhancer /note="translational enhancer" /regulatory_class="enhancer" CDS 932..1450 /label=VN173 /note="N-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" promoter 1650..1752 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 1783..2085 /label=ccdB /note="CcdB, a bacterial toxin that poisons DNA gyrase" regulatory 2155..2365 /label=CaMV 35S terminator /note="CaMV 35S terminator" /regulatory_class="terminator" primer_bind complement(2387..2403) /label=SK primer /note="common sequencing primer, one of multiple similar variants" promoter complement(2440..2458) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(2479..2495) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(2503..2519) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(2527..2558) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(2573..2594) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." misc_feature 2833..2857 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" rep_origin complement(2948..3536) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3640..4452) /label=KanR /note="aminoglycoside phosphotransferase" rep_origin 4743..5178 /label=pSa ori /note="origin of replication from bacterial plasmid pSa" misc_feature 5311..5333 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA (truncated)" terminator complement(5357..5608) /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" CDS complement(5651..6442) /label=NeoR/KanR /note="aminoglycoside phosphotransferase" promoter complement(6476..6655) /label=NOS promoter /note="nopaline synthase promoter" promoter 6925..6943 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 6969..6985 /label=KS primer /note="common sequencing primer, one of multiple similar variants"
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