pTS7 vector (V016044)

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LOCUS       62056_21590        5330 bp DNA     circular SYN 09-MAY-2021
DEFINITION  Cloning vector pTS7, complete sequence.
ACCESSION   MW835297
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Schnabel T.
  TITLE     Engineering post-translational regulation of glutamine synthetase 
            for controllable ammonia production in the plant-symbiont A. 
            brasilense
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Schnabel T.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2021) Bioengineering, Stanford University, 443 Via
            Ortega, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 5330)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-MAR-2021) Bioengineering, Stanford University, 443 Via Ortega, 
            Stanford, CA 94305, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..632)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(725..1870)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     regulatory      complement(1871..2160)
                     /label=pOri
                     /note="pOri"
                     /regulatory_class="promoter"
     regulatory      2243..2355
                     /label=pKan
                     /note="pKan"
                     /regulatory_class="promoter"
     oriT            complement(2243..2294)
                     /direction=LEFT
                     /note="oriT"
     CDS             2362..3153
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    3180..3240
                     /label=multiple cloning site
                     /note="multiple cloning site"
     oriT            complement(3332..3441)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        3613..3717
                     /label=AmpR promoter
     CDS             3718..4575
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4591..5179
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"