pKK50 vector (V016043)

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LOCUS       62056_13470        9065 bp DNA     circular SYN 15-FEB-2020
DEFINITION  Expression vector pKK50, complete sequence.
ACCESSION   MN811100
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   Krawczyk K, Scheller L, Kim H, Fussenegger M.
  TITLE     Rewiring of endogenous signaling pathways to genomic targets for 
            therapeutic cell reprogramming
  JOURNAL   Nat Commun 11 (1), 608 (2020)
  PUBMED    32001704
REFERENCE   2  (bases 1 to 9065)
  AUTHORS   Krawczyk KK.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2019) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 
            4058, Switzerland
REFERENCE   3  (bases 1 to 9065)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "608"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-DEC-2019) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 4058, 
            Switzerland"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(247..832)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1006..1863)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1864..1968)
                     /label=AmpR promoter
     enhancer        2234..2613
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2614..2817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        2862..2880
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2928..3325
                     /gene="GEAR-NFAT"
                     /label=MS2
                     /note="MS2"
     CDS             4371..4397
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             4401..4427
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             4434..4460
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     misc_feature    5739..5762
                     /gene="GEAR-NFAT"
                     /label=DNA binding
                     /note="DNA binding"
     CDS             5871..6587
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    6664..6888
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6934..7362
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7376..7705
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7772..8563
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    8740..8873
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8910..8926)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(8934..8950)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8958..8988)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9003..9024)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."