pT7RNAP-Q737P vector (V016035)

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LOCUS       V016035                 5693 bp    DNA     circular SYN 28-JUL-2021
DEFINITION  Exported.
ACCESSION   V016035
VERSION     V016035
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Becker K, Meyer A, Roberts TM, Panke S.
  TITLE     Plasmid replication based on the T7 origin of replication requires a
            T7 RNAP variant and inactivation of ribonuclease H
  JOURNAL   Nucleic Acids Res (2021) In press
   PUBMED   34255845
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Becker K.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) D-BSSE, ETHZ, Mattenstrasse 26, Basel, BS
            4058, Switzerland
REFERENCE   3  (bases 1 to 5693)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res (2021) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-JUN-2021) D-BSSE, ETHZ, Mattenstrasse 26, Basel, BS 4058,
            Switzerland"
FEATURES             Location/Qualifiers
     source          1..5693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        192..220
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    228..244
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             287..2935
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     CDS             complement(2958..3749)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(4237..5184)
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     rep_origin      complement(5232..5454)
                     /direction=LEFT
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"