pSin-Zap70-biosensor(SREYACI) vector (V016031)

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LOCUS       V016031                 9360 bp    DNA     circular SYN 28-JUL-2021
DEFINITION  Exported.
ACCESSION   V016031
VERSION     V016031
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Liu L, Limsakul P, Wang Y.
  TITLE     Integration of FRET and Sequencing to Engineer Kinase Biosensors
            from Mammalian Cell Libraries
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9360)
  AUTHORS   Liu L, Limsakul P, Wang Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2021) Department of Bioengineering, Institute of
            Engineering in Medicine, University of California, San Diego, 9500
            Gilman Drive 0435, La Jolla, CA 92093-0435, USA
REFERENCE   3  (bases 1 to 9360)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-JUL-2021) Department of Bioengineering, Institute of Engineering
            in Medicine, University of California, San Diego, 9500 Gilman Drive
            0435, La Jolla, CA 92093-0435, USA"
FEATURES             Location/Qualifiers
     source          1..9360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             117..750
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    797..922
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1415..1648
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1833..1877
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2026..2067
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2164..2281
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2409..3587
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3610..4293
                     /label="ECFP"
                     /note="enhanced CFP"
     misc_feature    4681..5394
                     /note="YPet; receptor"
     misc_feature    5476..6062
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             6082..6678
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             6884..7117
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7274..7498
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     promoter        7594..7698
                     /label="AmpR promoter"
     CDS             7699..8556
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8730..9318
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"