pLCB1 vector (V016028)

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LOCUS       62056_13735        7938 bp DNA     circular SYN 13-MAY-2019
DEFINITION  Cloning vector pLCB1, complete sequence.
ACCESSION   MK176919
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Li L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2018) Key Laboratory of Synthetic Biology, 
            Institute of Plant Physiology and Ecology, 300 Feng Lin Road, 
            Shanghai, Shanghai 200032, China
REFERENCE   2  (bases 1 to 7938)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2018) Key Laboratory of Synthetic Biology, Institute of 
            Plant Physiology and Ecology, 300 Feng Lin Road, Shanghai, Shanghai 
            200032, China"
FEATURES             Location/Qualifiers
     source          1..7938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             220..2004
                     /codon_start=1
                     /transl_table=11
                     /product="phiBT1 integrase"
                     /label=phiBT1 integrase
                     /protein_id="QCO69769.1"
                     /translation="MSPFIAPDVPEHLLDTVRVFLYARQSKGRSDGSDVSTEAQLAAGR
                     ALVASRNAQGGARWVVAGEFVDVGRSGWDPNVTRADFERMMGEVRAGEGDVVVVNELSR
                     LTRKGAHDALEIDNELKKHGVRFMSVLEPFLDTSTPIGVAIFALIAALAKQDSDLKAER
                     LKGAKDEIAALGGVHSSSAPFGMRAVRKKVDNLVISVLEPDEDNPDHVELVERMAKMSF
                     EGVSDNAIATTFEKEKIPSPGMAERRATEKRLASIKARRLNGAEKPIMWRAQTVRWILN
                     HPAIGGFAFERVKHGKAHINVIRRDPGGKPLTPHTGILSGSKWLELQEKRSGKNLSDRK
                     PGAEVEPTLLSGWRFLGCRICGGSMGQSQGGRKRNGDLAEGNYMCANPKGHGGLSVKRS
                     ELDEFVASKVWARLRTADMEDEHDQAWIAAAAERFALQHDLAGVADERREQQAHLDNVR
                     RSIKDLQADRKAGLYVGREELETWRSTVLQYRSYEAECTTRLAELDEKMNGSTRVPSEW
                     FSGEDPTAEGGIWASWDVYERREFLSFFLDSVMVDRGRHPETKKYIPLKDRVTLKWAEL
                     LKEEDEASEATERELAAL"
     primer_bind     2499..2515
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(2565..2661)
                     /regulatory_class="promoter"
     primer_bind     complement(2682..2698)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2706..2722)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2730..2760)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2775..2796)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3084..3672)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             3925..4725
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     CDS             complement(5162..5530)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(5563..5672)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(5910..5921)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     protein_bind    5992..6091
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     CDS             6101..7915
                     /label=phage phi-C31 integrase
                     /note="integrase from phage phi-C31"