pKK136 vector (V016017)

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LOCUS       V016017                 7190 bp    DNA     circular SYN 15-FEB-2020
DEFINITION  Exported.
ACCESSION   V016017
VERSION     V016017
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7190)
  AUTHORS   Krawczyk K, Scheller L, Kim H, Fussenegger M.
  TITLE     Rewiring of endogenous signaling pathways to genomic targets for
            therapeutic cell reprogramming
  JOURNAL   Nat Commun 11 (1), 608 (2020)
   PUBMED   32001704
REFERENCE   2  (bases 1 to 7190)
  AUTHORS   Krawczyk KK.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2019) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel
            4058, Switzerland
REFERENCE   3  (bases 1 to 7190)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "608"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-DEC-2019) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 4058,
            Switzerland"
FEATURES             Location/Qualifiers
     source          1..7190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             128..919
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    1096..1229
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1266..1282)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1290..1306)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1314..1344)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1359..1380)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1668..2253)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2427..3284)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3285..3389)
                     /label="AmpR promoter"
     rep_origin      3495..3630
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     misc_feature    3668..4024
                     /gene="GEAR SMAD2"
                     /label="MCP"
                     /note="MCP"
     CDS             4042..4755
                     /label="YPet"
                     /note="yellow fluorescent protein optimized for FRET with
                     CyPet (Nguyen and Daugherty, 2005)"
     CDS             4768..6165
                     /gene="SMAD2"
                     /label="Mothers against decapentaplegic homolog 2"
                     /note="Mothers against decapentaplegic homolog 2 from Homo
                     sapiens. Accession#: Q15796"
     polyA_signal    6210..6434
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6480..6908
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6922..7190
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"