FruR_I96R vector (V016016)

MSDS

Basic Vector Information

Vector Name:: FruR_I96R

Antibiotic Resistance:: Chloramphenicol

Length:: 3852 bp

Type:: Cloning vector

Replication origin:: p15A ori

Source/Author:: Groseclose TM, Rondon RE, Herde ZD, Aldrete CA

FruR_I96R vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

FruR_I96R vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V016016                 3852 bp    DNA     circular SYN 10-SEP-2020
DEFINITION  Exported.
ACCESSION   V016016
VERSION     V016016
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Groseclose TM, Rondon RE, Herde ZD, Aldrete CA, Wilson CJ.
  TITLE     Engineered systems of inducible anti-repressors for the next
            generation of biological programming
  JOURNAL   Nat Commun 11 (1), 4440 (2020)
   PUBMED   32895374
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Groseclose TM, Rondon RE, Herde ZD, Aldrete CA, Wilson CJ.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3852)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "4440"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-FEB-2020) Chemical "
FEATURES             Location/Qualifiers
     source          1..3852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        596..673
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             674..1675
                     /gene="cra"
                     /label="Catabolite repressor/activator"
                     /note="Catabolite repressor/activator from Escherichia coli
                     (strain K12). Accession#: P0ACP1"
     protein_bind    1808..1829
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      2020..2564
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     promoter        3090..3192
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3193..3849
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"

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