pRD2 vector (V016009)

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LOCUS       62056_18770        5055 bp DNA     circular SYN 09-FEB-2019
DEFINITION  Expression vector pRD2, complete sequence.
ACCESSION   MH298521
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Knopp M.
  TITLE     De novo emergence of peptides conferring antibiotic resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Knopp M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2018) Medical Biochemistry and Microbiology, 
            Uppsala University, Husargatan 3, Uppsala 75123, Sweden
REFERENCE   3  (bases 1 to 5055)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-MAY-2018) Medical Biochemistry and Microbiology, Uppsala 
            University, Husargatan 3, Uppsala 75123, Sweden"
FEATURES             Location/Qualifiers
     source          1..5055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      22..67
                     /label=lux terminator
                     /note="lux terminator"
                     /regulatory_class="terminator"
     CDS             complement(80..937)
                     /label=AmpR
                     /note="beta-lactamase"
     regulatory      complement(958..997)
                     /label=predicted promoter
                     /note="predicted promoter"
                     /regulatory_class="promoter"
     regulatory      complement(977..1016)
                     /label=predicted promoter
                     /note="predicted promoter"
                     /regulatory_class="promoter"
     terminator      1037..1064
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     regulatory      1091..1149
                     /label=T21 terminator
                     /note="T21 terminator"
                     /regulatory_class="terminator"
     protein_bind    1179..1195
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    1232..1253
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     regulatory      1254..1265
                     /regulatory_class="ribosome_binding_site"
     terminator      complement(1329..1360)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     regulatory      1408..1448
                     /label=terminator
                     /note="terminator"
                     /regulatory_class="terminator"
     terminator      complement(1469..1503)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1570..2988)
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     regulatory      complement(3012..3046)
                     /label=PsacB-J23113
                     /note="PsacB-J23113"
                     /regulatory_class="promoter"
     rep_origin      3064..3608
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        3886..3963
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3964..5043
                     /label=lacI
                     /note="lac repressor"