pKO403-lacZ-Cm vector (V016006)

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LOCUS       V016006                 4305 bp    DNA     circular SYN 01-SEP-2021
DEFINITION  Exported.
ACCESSION   V016006
VERSION     V016006
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Hend A, Yuka O, Tomoya K, Kouta S, Izumi N, Tohru S.
  TITLE     Bifidobacterium-E. coli shuttle vector series, pKO403, for metabolic
            engineering
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Hend A, Yuka O, Tomoya K, Kouta S, Izumi N, Tohru S.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2021) Contact:Altaib Hend Gifu University, Applied
            Biological Science; 1-1Yanagido, Gifu, Gifu 501-1193, Japan
REFERENCE   3  (bases 1 to 4305)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-AUG-2021) Contact:Altaib Hend Gifu University, Applied
            Biological Science; 1-1Yanagido, Gifu, Gifu 501-1193, Japan"
FEATURES             Location/Qualifiers
     source          1..4305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..11
                     /note="SapI site; three base pairs of tag sequence is
                     included"
     primer_bind     334..350
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(354..410)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(420..436)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(444..460)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(468..498)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(513..534)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    638..648
                     /note="SapI site; three base pairs of tag sequence is
                     included"
     misc_feature    662..2286
                     /label="Ori-Ts"
                     /note="Ori-Ts"
     gene            1288..1978
                     /gene="repB"
                     /label="repB"
     misc_feature    1288..1978
                     /gene="repB"
     misc_feature    2301..2534
                     /label="hup terminator"
                     /note="hup terminator"
     rep_origin      complement(2617..3205)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    3363..3552
                     /label="hup promoter"
                     /note="hup promoter"
     CDS             3553..4200
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_feature    4204..4263
                     /label="putative terminator"
                     /note="putative terminator"