pJL04 vector (V016003)

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LOCUS       62056_13190        8121 bp DNA     circular SYN 02-NOV-2020
DEFINITION  Cloning vector pJL04, complete sequence.
ACCESSION   MT370811
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8121)
  AUTHORS   Jie J, Chu X, Li D, Luo Z.
  TITLE     A set of shuttle plasmids for gene expression in Acinetobacter 
            baumannii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8121)
  AUTHORS   Jie J, Chu X, Li D, Luo Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2020) First Hospital, Jilin University, Xi'an 
            Road, Changchun, Jilin 130062, China
REFERENCE   3  (bases 1 to 8121)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-APR-2020) First Hospital, Jilin University, Xi'an Road, 
            Changchun, Jilin 130062, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(530..1060)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(1249..1277)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        complement(1295..1399)
                     /label=AmpR promoter
     terminator      complement(1435..1462)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             complement(1466..2545)
                     /label=lacI
                     /note="lac repressor"
     regulatory      complement(2546..2745)
                     /label=derived from pRpoD
                     /note="derived from pRpoD"
                     /regulatory_class="promoter"
     protein_bind    2752..2768
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2938..2961
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     rep_origin      complement(3097..3685)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      5084..6419
                     /label=oriV Acb
                     /note="oriV Acb"
                     /regulatory_class="replication_regulatory_region"